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T细胞依赖性抗体反应检测方法研究进展 被引量：2

Progress in the study of T cell-dependent antibody response
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摘要 T细胞依赖性抗体反应(TDAR)是检测免疫功能的主要试验技术。根据使用抗原及抗原特异性抗体检测方法的不同,TDAR方法包括绵羊红细胞(SRBC)作为抗原的空斑形成细胞(PFC)方法、SRBC作为抗原的ELISA方法和钥孔戚血蓝素(KLH)作为抗原的ELISA方法。KLH作为抗原比SRBC稳定、易标准化且容易获得,ELISA检测方法操作简便、样本可保存,便于整合到临床前毒理学研究中。本文综述了TDAR检测方法对免疫毒性的预测作用及其研究进展。 T cell-dependent antibody response （TDAR） is one of the main tests for evaluation of immune function. TDAR consists of 3 types of assays according to the different immunogens and detection methods of antigen specific antibody： SRBC-PFC assay, SRBC-ELISA assay and KLH-ELISA assay. KLH is easily available as an immunogen because of its good quality and standardization. ELISA method makes the operation easy and can be integrated into the preclinical toxicology studies conveniently. The prediction for the immunotoxicity of TDAR and the progress of the test are reviewed in this article.
作者 霍艳 艾文超 苗玉发 沈连忠 李波
机构地区 中国食品药品检定研究院
出处 《中国新药杂志》 CAS CSCD 北大核心 2011年第17期1620-1623,1672,共5页 Chinese Journal of New Drugs
基金 国家“重大新药创制”科技重大专项(2008ZX09305-002) 国家科技支撑计划课题(2008BAI54B01)
关键词 T细胞依赖性抗体反应 钥孔戚血蓝素 绵羊红细胞 免疫毒性 T cell-dependent antibody response （TDAR） keyhole limpet hemocyanin （KLH） sheep red blood cell （SRBC） immunotoxicity
分类号 R965.3 [医药卫生—药理学]
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参考文献15

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二级参考文献17

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中国新药杂志
2011年 第17期

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