猪脂肪干细胞与脱细胞真皮基质的生物相容性

Biocompatibility of procine adipose derived stem cells and acellular dermal matrix

背景:脱细胞真皮基质已广泛应用于器官或组织缺损的修补。目的:观察猪脂肪源性干细胞与脱细胞真皮基质的相容性。方法:酶消化法原代培养猪脂肪源性干细胞,利用流式细胞仪和多向诱导分化方法鉴定脂肪干细胞,将其与脱细胞真皮基质共培养。结果与结论:实验成功培养出猪脂肪源性干细胞,流式细胞仪检测结果显示阳性表达CD44和CD105,不表达CD34和CD45;在诱导培养基条件下,可向脂肪细胞、成骨细胞分化。苏木精-伊红染色及扫描电镜发现干细胞能在脱细胞真皮基质表面黏附生长。脂肪源性干细胞与脱细胞真皮基质具有良好的相容性,将两者体外复合培养后,有望将复合物植入体内进行缺损组织、器官的修复。

BACKGROUND：Acellular dermal matrix has been widely used as a repair material in organ and tissue repair.OBJECTIVE：To investigate the biocompatibility of procine adipose derived stem cells and acellular dermal matrix.METHODS：Porcine adipose derived stem cells were obtained and cultured with collagenase digestion method.Flow cytometric analysis and adipogenic,octeogenic differentiation analysis were used to identify the stem cells.RESULTS AND CONCLUSION：The stem cells isolated from subcutaneous adipose tissue were positive for CD44 and CD105,negative for CD34 and CD45.After cultured in mesenchymal stem cell adipogenic and octeogenic differentiation basal medium respectively for 3 weeks,the cells were positive for oil red O staining and alizarin bordeaux staining.Both hematoxylin-eosin staining and scanning electron microscopy analyses confirmed that adipose derived stem cells could adhere to acellular dermal matrix.Adipose derived stem cells have good biocompatibility with acellular dermal matrix.Adipose derived stem cells-seeded acellular dermal matrix can be used in tissue engineering as a mechanical reconstructive agent and as a biolayer of stem cells for targeted delivery.