注射用七叶皂苷钠联合脐带间充质干细胞移植改善脑梗死大鼠神经功能

Aescine sodium combined with umbilical cord mesenchymal stem cells transplantation for treatment of neurological function in rats with cerebral infarction

背景:单纯的脐带间充质干细胞移植修复受损脑组织的作用并不十分理想。目的:观察脐带间充质干细胞移植联合注射用七叶皂苷钠治疗大鼠脑梗死的效果。方法:应用线栓法建立大鼠大脑中动脉阻塞模型,随机分为对照组、细胞移植组、七叶皂苷钠+细胞移植组,分别尾静脉注射细胞培养液、1×1010L-1脐带间充质干细胞悬液、尾静脉1×1010L-1脐带间充质干细胞悬液同时经腹腔注射七叶皂苷钠5mg/(kg·d),连续5d。结果与结论:移植后1周,七叶皂苷钠+细胞移植组大鼠神经功能障碍评分低于细胞移植组及对照组(P<0.05);七叶皂苷钠+细胞移植组大鼠脑梗死周围组织AQP9及AQP4mRNA的表达低于细胞移植组,却高于对照组(P<0.05);七叶皂苷钠+细胞移植组CM-Dil阳性细胞和神经元数量多于细胞移植组及对照组(P<0.05)。提示脐带间充质干细胞移植联合注射用七叶皂苷钠治疗大鼠脑梗死可明显改善大鼠的神经功能。

BACKGROUND：The effect of single umbilical cord derived mesenchymal stem cells（UC-MSCs） transplantation repair damaged brain tissue is not very satisfactory.OBJECTIVE：To investigate the effect of UC-MSCs transplantation combined with aescine sodium on the treatment of cerebral infarction in rats.METHODS：The models of cerebral infarction were made by middle cerebral artery occlusion（MCAO）.Rats were divided randomly into control group,UC-MSCs group and Aescine sodium ＋ UC-MSCs group.The concentration of 1×1010/L UC-MSCs suspension or the concentration of 1 mL cell culture fluid was injected into MCAO models through caudal vein,at the same time,5 mg/（kg？d） aescine sodium aqueous solution or normal saline was injected into MCAO models through abdominal cavity,for 5 days.RESULTS AND CONCLUSION：The neurological dysfunction scores in aescine sodium ＋ UC-MSCs group were significantly lower than those in UC-MSCs group and control group at 1 week after transplantation（P〈0.05）.RT-PCR approved that the expression of AQP4 and AQP9 mRNA was highest in control group,a few in UC-MSCs group and lowest in aescine sodium ＋ UC-MSCs group（P〈0.05）.The number of CM-Dil-positive cells and neurons in aescine sodium ＋ UC-MSCs group was higher than that in UC-MSCs group and control group（P〈0.05）.The results suggested that UC-MSCs transplantation combined with aescine sodium can significantly improve neurological function of rats with cerebral infarction.