HPV16E6的表达与宫颈鳞癌发病的相关性研究

Study on the Correlation between Expression of HPV16E6 and Cervical Squamous Carcinoma

目的研究人乳头瘤病毒16E6(HPV16E6)蛋白在宫颈鳞癌中的表达及意义。方法应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接法观察40例宫颈鳞癌、30例宫颈上皮内瘤样病变(C IN)和30例正常宫颈组织中HPV16 E6的表达。结果 HPV16E6在正常宫颈组织、C IN和宫颈鳞癌组织中的阳性表达率分别为:13.3%、53.3%、82.5%,各组间表达率比较,差异有统计学意义(P<0.01)。HPV16E6蛋白阳性表达在宫颈鳞癌病理分级、临床分期中的比较,差异无统计学意义(P>0.05)。结论 HPV16E6在宫颈鳞癌的发生发展中起重要作用,可能是宫颈鳞癌发病机制之一。对HPV16E6蛋白的致病机制进一步研究,有助于揭示宫颈癌的发病机制,可能用于临床宫颈癌诊断、预防和基因治疗。

Objective To study human papillomavirus16 E6（HPV16E6） expression and their significance in cervical squamous carcinomas（CSC）.Methods IImmunohistochemical staining for HPV16E6 was performed by SP methods in 40 cases of CSC,30 cases of cervical intraepithelial neoplasias（CIN）,and 30 cases of normal cervical epithelium.Results he expression of HPV16E6 in 13.3% of normal cervical epithelium,53.3% of CINs,and 82.5% of the CSC were found,which were significantly statistically different（P0.001）.No statistical difference of the expression of HPV16E6 in different degrees of and clinical stages of CSC were observed（P0.05）.Conclusion The high detection rate of HPV16E6 in the CSC indicates it plays an important role in CSC development,which might be one of the pathogenesises（or might be the causes of CSC）.The results of the study of HPV16E6′s biological effects on CSC′ development and its interaction in CSC can help reveal the pathogenesis of CSC.The factor could be the potential CSC clinical diagnosis indicator and useful in CSC′s precaution and genetic therapy.