黄芪注射液和维生素E联合使用对大鼠肾缺血再灌注损伤的影响

Effect of Astragalus Injection Plus Vitamin E on Renal Ischemia Reperfusion Injury in Rats

目的观察黄芪注射液对大鼠肾脏缺血再灌注损伤的保护作用及机制。方法将SD大鼠随机分为假手术组、模型组、黄芪注射液组、维生素E组、黄芪+维生素E组,每组15只,采用切除右肾、动脉夹夹闭左肾动脉1h后松开恢复血流方法复制肾缺血再灌注损伤动物模型。黄芪注射液组、维生素E组分别于术前1h腹腔注射黄芪注射液10mg/kg或维生素E100mg/kg,黄芪+维生素E组同时给予上述两种药物。于缺血1h,再灌注1h、24h分别检测各组血清中尿素氮(BUN)、肌酐(Cr)含量、尿液中肾损伤分子-1(KIM-1)、6-乙酰-β-D-氨基葡萄糖酐酶(NAG)、肾组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)水平,并观察肾组织HE染色病理改变。结果与模型组比较,各治疗组血清BUN、Cr水平均显著降低(P<0.05或P<0.01),尿液KIM-1、NAG水平均显著降低(P<0.05或P<0.01),肾组织匀浆SOD活力升高、MDA含量降低(P<0.05或P<0.01),各治疗组之间比较差异有统计学意义(P<0.05)。肾组织切片镜下可见模型组大量肾小管上皮细胞肿胀、坏死,部分肾小管腔内可见蛋白性液体、红细胞或细胞碎片,治疗组肾组织病变减轻较明显。结论黄芪注射液和维生素E均可以增强SOD活性,清除氧自由基,减轻脂质过氧化作用,对缺血再灌注肾脏具有保护作用。黄芪注射液作用优于维生素E,二者联用效果更好。

Objective To observe the protective effect and mechanism of Astragalus Injection and vitamin E on renal ische- mia reperfusion injury in rats. Methods The SD rats were randomized into five groups： sham operated-group, model group, Astragalus group, vitamin E group, and Astragalus plus vitamin E group, with 15 in each. The ischemia-reperfusion injtiry models were made by restoring blood flow after removal 0f right kidney and clipping the left renal artery for an hour with artery clip. The Astragalus group and vitamin E group were injected Astragalus injectio 10mg/kg and vitamin E 100mg/kg respective- ly one hour before operation, and the Astragalus plus vitamin E group was treated with both simultaneously. The levels of ser- um blood urea nitrogen （BUN）, creatinine （Cr）, urinary kidney injury molecule -1 （KIM-1）, 6-acetyl-13-D-glucosaminidase （NAG）, and the activity of superoxide dismutase （SOD） and malondialdehyde （MDA） of renal tissues of all groups were detec- ted one hour after ischemia, one hour and twenty-four hours after reperfusion respectively and dynamically, and the pathologi- cal changes in HE staining 0f renal tissues were observed as well. Results Compared with model group, the 3 treatment groups had significant decrease in the levels of serum BUN and. Cr, decrease in the levels of KIM-1 and NAG, increase in the activity of SOD, and decrease in the level of MDA（P.〈0.05 or P〈0.01）. The comparison of the 3 treatment groups showed significant difference （P〈0.05）. The pathological changes of the renal tissues existed in model group,i, e. a large number of renal tubular epithelial cells became swollen and necrosis, and there was liquid protein and red blood cells or cell fragments in the renal tubular cavity. The pathological changes of the renal tissues in the treatment groups were significantly relieved. Con- clusion Astragalus injection and vitamin E may enhance the activity of SOD, clear oxygen free radicals, and reduce lipid perox- idation, having a protective effect on ischemia-reperfusion kidney. Astragalus injection may be Superior to vitamin E in the pro- tective effect, and the combination of both is better in result.