摘要
目的：建立以HPLC法测定参南星口服液中人参皂苷Rg1含量的方法。方法：采用Thermo C18（200mm×4．6mm，5μm）柱，以乙腈：0．4％磷酸=20：80为流动相，检测波长：203nm，流速1．0ml·min^-1。结果：人参皂苷Rg1在0．4284—2．142μg范围内线性关系良好，r=0．9999，平均加样回收率为99．2％。结论：本方法操作简便、方法可靠，结果准确、灵敏度高，重复性好，适合于参南星口服液中人参皂苷Rg1的含量测定。
Objective： To establish a HPLC method for the determination of GinsenosideRg~ in Shennanxing Koufuye. Method： A Thermo C18（ 4.6mm × 200mm,5 μ m ）column was used. The mobile phase was acetonitrile： 0.4% phosphoric acid =20：80, the detecting wavelength was at 203nm and the flow rate was 1.0ml·min^-1.Result：The linear range of GinsenosideRgL was within 0.4284- 2.142 μ g. r =0.9999,the average recovery was 99.2%.Conclusion： This method has good repeatability, flexibility and high sensitivity. It can be used for quality control of Shennanxing Koufuye.
