苦豆子总碱对SW480细胞株及裸鼠移植瘤的影响

Effect of Total Alkaloid of Sophora alopecuroides on SW480 Cells and Balb/c Nude Mice Tumor Xenograft

目的:探讨苦豆子总碱(TASA)对SW480细胞株及裸鼠移植瘤的抑制作用及机制。方法:利用MTT法检测不同浓度TASA对SW480细胞的增殖抑制影响;Annexin V-FITC标记法检测SW480细胞凋亡;建立裸鼠移植性实体瘤SW480模型,观察TASA作用下移植性实体瘤的体积和质量,用荧光定量PCR法检测移植性实体瘤组织中Caspase-3、Caspase-9及BCL-2 mRNA的表达。结果:实验结果显示TASA对体外培养的SW480细胞增殖具有明显抑制作用,并可诱导其发生凋亡,其作用呈剂量和时间依赖性,48 h IC50=0.92 mg/mL;TASA高、低剂量组裸鼠移植性实体瘤SW480的体积和质量、BCL-2表达低于模型组,Caspase-3、Caspase-9表达则显著高于模型组,其抑瘤率分别达到57.3%、37.4%。结论:TASA对SW480细胞株及裸鼠移植瘤均有抑制作用,其机制可能与诱导凋亡有关。

Objective：To study the anti-tumor effect of total alkaloid of Sophora alopecuroides（TASA） on human colon adenocarcinoma SW480 cells and Balb/c nude mice tumor xenograft.Methods：The effect of TASA on cell proliferation was assessed using MTT assay and the cell apoptosis was detected using Annexin V-FITC apoptosis assay.The nude mouse model bearing transplanted solid tumor SW480 was established.The changes of the volume and weight of the tumor were determined after treatment the mice with TASA.Fluorescence quantitative PCR was used to detect Caspase-3,Caspase-9 and BCL-2 mRNA expressions in the tumor.Results：TASA inhibited the proliferation of SW480 cells in a dose-and time-dependent manner.The apoptotic rate of cells was the best when the concentration of TASA was 0.92 mg/mL at 48 hours.The volume and weight of the tumor xenograft in TASA groups were decreased when compared with those of the control group.The results of RT-PCR showed that TASA activated the pro-apoptotic Caspase-3 and Caspase-9 and lowered expressions of BCL-2.Conclusion：TASA can inhibit the growth of SW480 cells and the growth of transplanted solid tumor of human SW480 cell line,the mechanism of which involves the effect of Caspase-3,Caspase-9 and BCL-2 expression.