红景天中红景天苷和多糖制备工艺研究

Study on Preparation of Salidroside and Polysaccharide in Rhodiola crenulata

目的:优选红景天中红景天苷和多糖的制备工艺。方法:通过水提醇沉法,采用L9(34)正交试验法,以浸膏得率、红景天苷含量、多糖含量为评价指标,考察加水量、煎煮时间、煎煮次数对水提取工艺的影响;以红景天苷含量、多糖含量为评价指标,考察药液浓度、药液含醇量对醇沉工艺的影响。结果:以煎煮3次,每次加8倍量水,煎煮2 h为最佳提取工艺,以药液浓缩至每1 mL相当于原生药0.4 g,加95%乙醇醇沉,使药液含醇量70%为最佳醇沉工艺。结论:优选工艺可为新药研究和综合利用红景天资源提供参考。

Objective：To optimize the preparation process of salidroside and polysaccharide in Rhodiola crenulata.Methods：Water-extraction and alcohol-precipitation was used.The effect of added water volume,decoction time and frequency on water-extracting process was investigated by L9（34） orthogonal design using extraction rate,content of salidroside and polysaccharide as the assessment indexes.The effect of liquor strength and ethanol concentration on alcohol-precipitating process was investigated as well,using content of salidroside and polysaccharide as the assessment indexes.Results：The optimum conditions for water-extracting process were as follows：decocting 3 times,and each time adding 8 fold of water and extracting 2 h.For alcohol-precipitating process,it was that liquor strength was 0.4 g/mL,and ethanol concentration in liquor was 70%.Conclusion：The optimized process has guidance for the development and comprehensive utilization of this plant.