果胶酶提取金丝桃素工艺研究

Study on the Enzyme Extraction Process of Hypericin by Pectinase

目的:探讨贯叶连翘中金丝桃素的酶提取法的最优酶及最佳工艺。方法:在果胶酶、木聚糖酶、β-葡聚糖酶、复合酶SPE-007A中筛选出最优酶,并以高效液相色谱法测定金丝桃素含量为指标,继考察浸提倍数、加酶量、pH值、温度、酶解时间等因素对提取工艺的影响,并用正交试验优选提取条件,从而得到最佳工艺。结果:最优酶为果胶酶,优选出的工艺为pH4.6、酶用量1.5%、酶解温度50℃、酶解时间5 h、酶解液浸提倍数15倍。结论:该工艺高效、稳定,可用于今后对贯叶连翘的研究。

Objective：To explore the best enzyme and optimal conditions for extracting Hypericin from Hypericum perforatum.Methods： Chose the best enzyme from Pectinase,Xylanase,Glucanase,β-Glucanase and Enzyme（SPE-007A）.The effeet of solid-liquid ratio enzyme dosage,PH,temperature and the extraction time were investigated by L9（34） orthogonal design using extraction rate and the content of Hypericin as assessment index.Results： The best enzyme was Pectinase and the optimum extraction process was as follows：PH 4.6,enzyme dosage 1.5%,temperature 50 ℃,extraction time 5 h,liquid-solid ratio 15 times.Conclusion： This method is efficient and stable.It could be used in the future research of Hypericum perforatum.