摘要
建立测定猪肠衣中磺胺二甲基嘧啶残留量的ELISA分析法。方法检测低限为5μg/kg;在5μg/kg、20μg/kg和100μg/kg三个添加水平进行加标回收试验,回收率分别为82.0%-96.0%、87.5%-102.0%和83.9%-105%;重复性试验:CV%分别为5.3%、5.3%和8.2%;重现性试验:CV%分别为8.0%、7.9%和7.5%。本方法灵敏度、准确度和精密度均符合残留分析质量控制的要求,适用于猪肠衣中磺胺二甲基嘧啶残留量的快速检测。
[Objective] To develop an enzyme immunoassay method for the detection of sulphodimides(SM2) residues in aquatic products.[Results] The limit of detection(LOD) was 5 μg/kg;At the spiked levels of 5 μg/kg,20μg/kg and 100μg/kg,the mean recovery of this method were 82.0%-96.0%,87.5%-102.0% and 83.9%-105%,respectively,with the CV% for the repeatability being 5.3%,5.3% and 8.2%,and the CV% for the reproducibility being 8.0%,7.9% and 7.5%.[Conclusions] The sensitivity,accuracy and precision of the method all accorded with the guide for quality control of residues analysis.It is suitable for the rapid detection of SM2 residues in hog casing.
出处
《检验检疫学刊》
2011年第4期21-22,52,共3页
Journal of Inspection and Quarantine