P120连环素在乳腺浸润性导管癌中的表达与临床病理意义

Expression and clinicopathological significance of P120 in mammary invasive ductal cancer

目的:探讨P120在乳腺浸润性导管癌中的表达及其与临床病理特征的关系。方法:采用免疫组化方法检测84例乳腺浸润性导管癌组织中ER、PR、c-erbB-2及P120的表达情况;84例乳腺癌病例中有43例同时取相应新鲜肿瘤组织和癌旁的正常组织,采用RT-PCR方法检测其P120的表达情况。结果:84例乳腺浸润性导管癌中P120蛋白在细胞膜正常表达率为39.3%。43例乳腺癌术中留取的新鲜肿瘤组织中P120 mRNA阳性表达率为37.2%。P120在乳腺浸润性导管癌与癌旁正常组织中的表达差异有统计学意义(P<0.01)。P120表达与淋巴结转移、临床分期及c-erbB-2表达均有明显相关性(P<0.05),P120蛋白表达与患者年龄、肿瘤大小、肿瘤组织学分级及ER、PR表达间均未见明显相关性(P>0.05)。结论:P120的异常表达可能反映了乳腺浸润性导管癌的侵袭性生物学行为。

Objective： To investigate the relationship between the expression of P120 and clinicopathological characteristics in mammary invasive ductal cancer.Methods： Immunohistochemistry was used to detect the expression of ER,PR,c-erbB-2,and P120 oncoprotein in 84 cases of mammary invasive ductal carcinoma.RT-PCR assay was used to detect the expression of P120 mRNA in 43 cases（from above 84 cases） of breast cancer and paraneoplastic tissues.Results： The positive rate of P120 oncoprotein expression were 39.3% in 84 cases of mammary carcinoma.The positive rate of P120 mRNA expression was 37.2% in 43 cases of breast cancer with fresh specimens obtained from surgical operations.The significant differences were seen between the expression of P120 in breast cancer and in paraneoplastic tissues（P〈0.01）.The aberrant expression of P120 oncoprotein was positively related to lymph node metastases,clinical advanced stage and positive c-erbB-2（P〈0.05）,but not to age,tumor size,histological grading,and ER,PR（P〉0.05）.Conclusion： The aberrant expression of P120 probably indicates the aggressive biological behavior of mammary invasive ductal carcinoma.