20(R)-原人参二醇酸水解条件的工艺研究

Study on the Technology of Hydrolysis Process of 20(R)-PPD with Hydrochloric Acid

目的:优化三七茎叶总皂苷的盐酸水解工艺,提高水解产物20(R)-原人参二醇[20(R)-protopanaxadiol,20(R)-PPD]的产率。方法:采用正交试验设计,考察料液比、盐酸浓度、水解时间、水解温度对三七茎叶总皂苷水解过程的影响。结果:影响水解的主次因素依次是盐酸浓度>料液比>水解温度>水解时间,优选的最佳工艺为料液比1∶1;盐酸浓度17.1mol·L-1,水解时间90min,水解温度50℃。结论:采用优化后的水解条件能够提高PPD的转化率,适合工业化生产。

Objective： To optimize the hydrolysis technology of total saponins in Panax notoginseng with hydrochloric acid in order to increase the yield of 20 （R）-PPD. Methods： Orthogonal design was used to optimize the hydrolysis condition and four factors including solid/liquid ratio, concentration, reaction time and reaction temperature were investigated to study the process of hydrolysis. Results： The primary and secondary influence factors of this process were listed as follows ： concentration 〉 solid/liquid ratio 〉 temperature 〉 time and the optimization technology of hydrolysis process obtained was：the ratio of liquid to solid material at i： 1, the concentration of hydrochloric acid with 17.1 mol. L-1, and the reaction time and temperature were 1.5 h and 50℃ respectively. Conclusion： The yield of 20 （R）-PPD under the optimum hydrolysis condition was raised and this technology can be used for industrialization production.