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吉林地区丙型肝炎病毒基因分型及临床意义的研究 被引量：1

Genotyping and clinical significance of hepatitis C virus in Jilin
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摘要 目的了解吉林地区HCV基因分型情况,为HCV感染者的诊断和治疗提供重要的科学依据。方法荧光定量RT-PCR检测165例抗-HCV阳性血清标本,并对153例HCV-RNA阳性的血清标本应用基因芯片法进行HCV基因分型。结果 153例阳性标本中HCV1b型85例,HCV2a型63例,HCV1b/2a混合型5例。结论吉林地区HCV基因型主要为1b型,2a型次之,同时存在HCV1b/2a混合型。 Objective To investigate the distribution of hepatitis C virus（HCV） genotypes in Changchun and to provide information for the diagnosis and treatment of HCV.Methods Eighty-nine samples HCV-RNA positive from 165 samples anti-HCV positive sera by fluorescence quantitative RT-PCR were further genotyped by gene chip.Results Forty-nine sera was type 1b,63 sera was type 2a and 3 sera was 1b/2a mixed-type among 153 samples with HCV-RNA positive.Conclusion HCVlb is the most dominant genotype,HCV2a is the second and HCV1b/2a mixed-type also exists in Changchun.
作者 李晓峰 马寅芙 杨广民
机构地区 吉林省人民医院
出处 《中国实用医药》 2011年第23期33-34,共2页 China Practical Medicine
关键词 丙型肝炎病毒 荧光定量RT-PCR 基因分型 Hepatitis C virus Fluorescence quantitative RT-PCR Genotype
分类号 R450 [医药卫生—治疗学]
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参考文献9

  • 1王文博,任浩.丙型肝炎病毒F蛋白在病毒复制和致病中的作用[J].中国生物制品学杂志,2011,24(3):349-353. 被引量：6
  • 2Liew M, Erali M, Page S, et al. Hepatitis C genotyping by denatu- ring high-performance liquid chromatography. Clztt Microbiol, 2004,42 ( 1 ) : 158-163.
  • 3Etsuro Oxito. HCV genotype/serotype. Japanese clinical, 2001,59 (7) :1356.
  • 4Verachai V, Phutiprawan T. Prevalence and genotypes of hepatitis C virus infection among drug addicts and blood donors in Thai- land. Southeast Asian J Trop Ned Public Health,2002,33 : 849 -851.
  • 5Mellor J, Walsh EA, Prescon LE, et al. Survey of type 6 group va- riants of hepatitis C virus in Southeast Asia by using a cow-based genotyping assay. J Clin Microbiol, 1996,34 (2) :417-423.
  • 6Maria M, Jordi G, Juan IE, et al. High-thoughput realtime reverse ranscription-PCR quantitation of HCVRNA. ] Clin Microbiol,1999, 37:327-332.
  • 7霍艳英,徐德忠,赵小宁,李如琳,刘蓬勃,王全楚,王歆.西安和郑州地区丙型肝炎患者的HCV基因分型[J].第四军医大学学报,2002,23(8):749-751. 被引量：84
  • 8蒋伟伦,顾士民,胡芸文,许河生,孙亚洲.上海地区丙型肝炎病毒基因分型的研究[J].中华肝脏病杂志,1999,7(1):29-30. 被引量：17
  • 9Kandi K, Kako M, Okamoto H. HCV genotypes in chronic hepatitis C and response to interferon. Lancet,1992,339:1543.

二级参考文献40

  • 1郭林生,汪先恩.中国HCV感染株的基因分型研究[J].肝脏病杂志,1993,1(3):152-156. 被引量：5
  • 2陆志檬,张东华,童葵塘,高健,白敬羽,徐伯忠.HCV基因型和病毒量与干扰素疗效的关系[J].中华传染病杂志,1995,13(2):65-68. 被引量：28
  • 3Sander C, Schneider R. Database of homology-derived protein structures and the structural meaning of sequence alignment [J]. Proteins, 1991, 9 (1) : 56-68.
  • 4Shesheer KM, Venkateswara RK, Mohammed HC, et al. Expression of alternate reading frame protein (F1) of hepatitis C virus in Escherichia coli and detection of antibodies for F1 in Indian patients [J]. Infect Genet Evol, 2008, 8 (3): 374-377.
  • 5Spengler U, Nattermann J. Immunopathogenesis in hepatitis C virus cirrhosis [J]. Clin Sci (Lond), 2007, 112 (3): 141-155.
  • 6Moriya K, Fujie H, Shintani Y, et al. The core protein of hepatitis C virus induces hepatocellular carcinoma in transgenic mice [J]. Nat Med, 1998, 4 (9): 1065-1067.
  • 7Fiorucci M, Boulant S, Fournillier A, et al. Expression of the alternative reading frame protein of hepatitis C virus induces cytokines involved in hepatic injuries [J]. J Gen Virol, 2007, 88 (Pt 4): 1149-1162.
  • 8Tsao ML, Chao CH, Yeh CT. Interaction of hepatitis C virus F protein-with prefoldin 2 perturbs tubulin cytoskeleton organization [J]. Biochem Biophys Res Commun, 2006, 348 ( 1 ) : 271-277.
  • 9Branch AD, Walewski JL, Gutierrez JA, et aL HCV alternate reading frame proteins (ARFPs) may be virulence factors that help the virus survive adverse conditions[J]. Hepatology, 2003, 38 : 468A-469A.
  • 10Wu WB, Shao SW, Zhao LJ, et al. Hepatitis C virus F protein up-regulates c-mye and down-regulates p53 in human hepatoma HepG2 cells [J]. Intervirology, 2007, 50 (5): 341-346.

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中国实用医药
2011年 第23期

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