发酵液中替考拉宁含量的HPLC法测定

HPLC determination on the content of Teicoplanin in fermented broth

目的建立用HPLC法测定替考拉宁含量的方法。方法采用C18柱(250 mm×4.6 mm,5μm),流动相为0.02 mol/L的磷酸缓冲液(pH6.0)-乙腈(75∶25),流速1.0 ml/min,检测波长为254 nm,进样量10μl。结果替考拉宁在500.0～5000.0μg/ml范围内线性关系良好(r=0.9988),平均回收率为100.2%(RSD=0.14%,n=6)。结论方法简便,结果准确,适用于该产品的质量控制。

Objective To establish the determination on the content of Teicoplanin in fermented broth.Methods An HPLC method was developed,C18column（250 mm×4.6 mm,5 μm） was used,and the mobile phase consisted of phosphate buffer（pH 6.0）and acetonitrile（75：25）at the flow rate of 1.0 ml/min.The detection wavelength was 254nm,and the injection volume was 10 μl.Results The linear range of Teicoplanin was 500.0~5000.0 μg/ml（r=0.9988）,and the average recovery was 100.2%（RSD=0.14%,n=6）.Conclusion This method is convenient and accurate,which is suitable for the quality control of this preparation.