摘要
由食品整治办发布的中国检验检疫科学院食品安全所提供的"乳与乳制品中动物水解蛋白鉴定—L(-)-羟脯氨酸含量测定"中试样的两种水解方法在实际操作中均有不同程度的缺陷,方法一采用的是已经作废的《GB 9695.23-90肉与肉制品L(-)-羟脯氨酸含量测定》中的水解方法,方法二有抽真空再充氮气以及需要110℃密封水解,操作复杂且危险。本文对试样的水解方法进行了改进,采用改进后的方法,加标回收率为84.8%,相对标准偏差为2.2%,检测限为0.001μg/mL,定量限为0.004μg/mL,该方法不仅简化了羟脯氨酸测定的操作步骤,并且准确可行,具有良好的准确度、精密度、检测限和定量限低等优点。
Two hydrolysis methods of samples in ＂Milk and dairy products in the identification of animal protein hydrolysate-determination of L（-） –hydroxyproline＂,which were published by Office of Food Regulation and provided by National Food Safety Information Center,have defect of different degree in actual experiments.The first method applied 〈GB 9695.23-90 Meat and meat products-Method for determination of L-hydroxyprolin Content〉,which has been abolished.The second method was dangerous and complicated,being involved in vacuumizing,filling nitrogen gas and sealed hydrolyzing at 110 °C.This paper improved the hydrolysis method of samples.With the improved method,the recovery rate of standard addition was 84.8%,the relative standard deviation was 2.2%,the detectability was 0.001μg/mL and the limit of quantitation was 0.004μg/mL.This approach not only simplifies the steps of operating in determination of hydroxyproline,but also is accurate and feasible.The method have good accuracy,precision,low detectability and limit of quantiation
出处
《现代科学仪器》
2011年第4期47-49,共3页
Modern Scientific Instruments
