1株高效反硝化聚磷菌的生物学特性研究

Biological Characteristics of Denitrifying Polyphosphate-accumulating Organisms

采用专性培养基,从稳定运行的A/O/A SBR反应器中分离得到1株高效的反硝化聚磷菌Q-hrb05.菌株Q-hrb05的16S rDNA序列登录GenBank,登录号为GU214826.分析了该菌株的胞外聚合物的成分,探讨了pH、温度和碳源对株菌的生长及脱氮除磷效能的影响.结果表明,菌株Q-hrb05为芽孢杆菌,胞外聚合物以蛋白质为主,约120.6 mg.mL-1;核酸23.05μg.mL-1,多糖极少且对磷酸盐无明显吸附作用,对磷的去除主要源于细胞内的吸收.当pH值为7,温度为30℃,碳源为乙酸钠时,最利于菌株生长,且同步脱氮除磷效果最佳.此时除磷率为88%,脱氮率为81%.

A denitrifying phosphate-accumulating organisms（DNPAOs）,which was called Q-hrb05,was isolated in the special medium from the anaerobic/aerobic/anoxic SBR reactor.Strain Q-hrb05 was identified by 16SrDNA gene analysis,and the accession number of 16SrDNA gene sequence of strain Q-hrb05 in GenBank was GU214826.Effects of the different pH values,temperature,carbon source of medium on nitrogen and phosphorus removal of strain Q-hrb05 were investigated.The result showed that strain Q-hrb05 belonged to Bacillus sp..Meanwhile,extracellular exopolymers of strain Q-hrb05 was based on protein,about 120.6 mg·mL-1,and it had 23.05 μg·mL-1 nucleic acid,but little polysaccharide.There was no significant adsorption of phosphate.So phosphorus removal was mainly due to intracellular uptake.And when pH value was kept as 7,temperature was kept as 30℃,and carbon source was kept sodium acetate,the highest nitrogen and phosphorus removal efficiency was achieved.Phosphorus uptake rate was averaged at 88%,and the denitrification rate reached 81%.