枣花蜜中抗氧化成分提取工艺优化

Optimization of Purification Process for DPPH Free Radical Scavenging Components from Acidic Aqueous Extract from Chinese Date Honey by Macroporous Resin Adsorption

研究树脂提取枣花蜜中抗氧化成分的方法,包括蜂蜜提取条件、树脂筛选;蜂蜜与树脂比例、吸附流速对吸附的影响;洗脱剂体积分数、解吸流速、体积对解吸的影响,以期为充分提取蜂蜜中抗氧化成分提供实验依据。通过未吸附物和解吸物中抗氧化成分对DPPH自由基清除率的比较,确定采用XAD-2树脂最佳吸附和解吸条件为蜂蜜与树脂比例1:1(m/m),吸附流速1.0mL/min,洗脱剂80%乙醇,解吸流速4.0mL/min,洗脱体积2BV。

Solvent extraction and purification by macroporous resin adsorption were used to acquire DPPH free radical scavenging components from Chinese date honey.Extraction solvent type,macroporous resin type,sample loading amount,sample loading flow rate,eluent type,elution flow rate and elution volume were optimized.Acidified ultrapure water（adjusted to pH 2 with HCl） was better solvent for DPPH free radical scavenging components in Chinese date honey than ultrapure water.Among four types of macroporous resin investigated,XDA-2 resin was found to be the most suitable choice.The optimal adsorption and desorption parameters of XDA-2 resin for purifying DPPH free radical scavenging components extracted from Chinese date honey of the same weight were sample loading flow rate of 1.0 mL/min,80% ethanol aqueous solution as the eluent,elution flow rate of 4.0 mL/min and elution volume of 2 BV.