青钱柳愈伤组织总黄酮碱溶酸沉提取工艺

Optimization of Alkali Extraction and Acid Precipitation Process for Total Flavonoids from Cyclocarya paliurus Callus

目的:研究以碱溶酸沉法提取青钱柳愈伤组织中的总黄酮。方法:采用碱溶酸沉法,对影响青钱柳愈伤组织中总黄酮提取的pH值、固液比、提取时间、硼砂用量等因素进行系统研究。结果:青钱柳愈伤组织中总黄酮碱提酸沉的最佳提取工艺为:采用固液比1:20,碱提液pH9,硼砂含量2.5%,提取时间60min,酸沉pH4.5。结论:在最佳提取工艺下,青钱柳愈伤组织中总黄酮得率达到4.01%。

Objective： To optimize the alkali extraction and acid precipitation of total flavonoids from Cyclocarya paliurus callus.Methods： The effects of pH,material/liquid ratio,borax amount and extraction time on the extraction rate of total flavonoids from Cyclocarya paliurus callus.Results： The optimal extraction conditions were extraction solvent pH of 9.0,borax amount of 2.5%,extraction time of 60 min,and acid precipitation pH of 4.5.Conclusion： Under the optimal conditions,the extraction rate of total flavonoids was up to 4.01%.