KiSS-1、黏蛋白1和上皮钙黏附蛋白在乳腺癌组织中的表达及其与临床病理特征的关系

Expression of KiSS-1, mucins 1 and E-cadberin in breast cancer and their correlations with clinico- pathological features

目的探讨KiSS-1、黏蛋白1（MUC1）和上皮钙黏附蛋白（E．cad）在乳腺癌组织中的表达及其与临床病理特征的关系。方法应用免疫组织化学SP法检测106例乳腺癌组织中KiSS-1、MUC1和E-cad的表达，分析KiSS-1、MUC1和E-cad在癌组织表达的相关性及其与临床病理参数的关系。结果KiSS-1、MUC1和E-cad在乳腺癌组织中的表达与淋巴结转移（N0 58．97％／38．46％／74．36％：N，26．87％／61．19％／53．73％）和临床分期（I和Ⅱ60．66％／39．34％／70．49％；Ⅲ和Ⅳ8．89％／71．11％／48．89％）有关（r=-10．715／5．112／4．422和29．259／10．487／5．095，P均〈0．05），与患者年龄、绝经期、肿瘤T分期、M分期无明显相关（P值均〉0．05）。伴随着淋巴结转移和临床分期的增加，乳腺癌组织中KiSS-1和E-cad的表达下调或缺失，MUC1的表达则升高。随着乳腺癌组织分化程度的降低，E-cad（高分化75．00％；中分化66．67％；低分化28．58％）的表达下调或缺失（x2=12．391，P〈0．05），MUC1（高分化32．14％；中分化56．14％；低分化71．43％）的表达升高（X2=16．631，P〈0．05）。乳腺癌组织中KiSS-1和MUC1的表达呈负相关（r=-0．714，P〈0．05），和E．cad的表达呈正相关（r=0．763，P〈0．05）。结论KiSS．1、MUC1和E-cad的表达与乳腺癌临床分期和淋巴结的转移相关，其相互作用在乳腺癌细胞的黏附、侵袭和转移中起重要作用，KiSS-1可能作为一种肿瘤转移抑制蛋白发挥重要调节作用。

Objective To investigate the expression of KiSS-l, mucins 1 （ MUC1 ） and E-cadher- in （E-cad） in human breast cancer and their correlations with clinicopathologieal features. Methods The expression of KiSS-l, MUC1 and E-cad in 106 specimens of breast cancer was detected by immunohisto- chemistry, and their correlations to the clinicopathological features of breast cancer were analyzed. The cor- relations of KISS-1 expression to MUC1 and E-cad were analyzed by Spearman＇ s rank correlation. Results The expression of KiSS-l, MUC1 and E-cad had some relations with clinical stages （ Ⅰ and Ⅱ 60. 66%/ 39. 34%/70. 49% , Ⅲ and Ⅳ 8.89%/71.11%/48.89%）, lymph node metastasis （ NO 58. 97%/ 38. 46%/74. 36% ; N1 26. 87%/61.19%/53.73% ） of breast cancer （X2 = 10. 715, 5. 112, 4. 422 and 29. 259, 10. 487, 5. 095, P all 〈 0.05 ）, but they had no correlation with age, menopause, T stages and M stages （P all 〉0. 05）. The expression of KISS-1 and E-cad were decreased gradually with the progress of lymph node metastasis and clinical stages, and MUC1 expression increased. The expression of E-cad （well 75.00% ; moderate 66. 67% ; poor 28. 58% ） was decreased gradually with the progress of histological grade （X2= 12. 391 ,P 〈 0. 05）, and MUC1 （well 32. 14%; moderate 56. 14%; poor 71.43% ） expres- sion increased （X2 = 16. 631 ,P 〈0.05）. The negative correlation was observed between KISS-1 expression and MUC1 expression （r = -0. 714, P 〈 0. 05 ）, and positive correlation between KISS-1 and E-cad in breast cancer （r =0. 763,P 〈 0. 05 ）. Conclusion The expressions of KISS-1, MUC1 and E-cad may re- flect the metastasis and clinical stages of human breast cancer. The interaction of these factors plays an important role in regulation of the invasion and metastasis of breast cancer ceils, and it is possible for KISS-1 to play an important role as a tumor metastasis inhibition protein.