高质量加工番茄基因组DNA提取方法的改进

The Improvement of Extraction Method for High-quality Genomic DNA from Processing Tomato

加工番茄在生长过程中会富积大量的多糖、蛋白质和其他次生代谢物质,采用文献报道的方法不易提取(高质量的)DNA或提取的DNA溶液容易降解。采用改进的CTAB法,提取加工番茄幼嫩叶片和老叶片的基因组DNA,经紫外分光光度计和凝胶电泳检验,提取到的DNA纯度高、完整性好,PCR扩增能得到明显的多态性谱带,可直接用于后续相关试验。

The processing tomato will accumulate large quantities of polysaccharides, protein and other secondary metabolites during it grow process, and it is difficult to extract high-quality DNA or the extracted DNA can be broken down easily with the reported DNA extraction method. In this paper an improved CTAB method was used to extract gcnomic DNA from young leaves and old leaves of processing tomato. The extracted DNA was detected by UV spectrophotometer and gel electrophoresis, the results showed that the extracted DNA had high purity and good integrity. The PCR amplification can get obvious polymorphic fragments of extracted DNA, so it can be directly used for subsequent experiments.