毛细管气相色谱法测定山楂中15种有机磷农药残留的研究

Determination of 15 Types of Organophosphorus Pesticide Residues in Hawthorn by Capillary Gas Chromatography

目的：建立山楂中15种有机磷农药残留量同时检测的分析方法。方法：样品以混合溶剂超声提取，Florisil固相萃取柱净化处理后，采用DB-5毛细管气相色谱柱分离样品，火焰光度检测器检测，外标法定量。结果：在1～10ng·g。添加范围内，15种有机磷农药的回收率为83．2％-90．8％；相对标准偏差（RSD）为3．1％～5．6％；最低检测限为0．05～0．1ng·g-1。（S／N=3）。结论：此方法简便、可靠，可用于山楂中的有机磷农药残留量测定。

This study aimed to establish the method for simultaneous determination of 15 types of organophosphorus pesticide residues in hawthorn （Crataegus p innatifida var. major）. After ultrasound-assisted extraction of sample from mixed solvent, the extract was purified by Florisil solid-phase clean-up column. Then, the extract was separated by capillary column and detected by flame photometric detector. Results showed that the average recovery of 15 types of organophosphorus pesticide residues spiked at levels of 1-10 ng·g-1. It ranged from 83.2% to 90.8%. The relative standard deviation （RSD） is 3.1%-5.6%. The detection limit for 15 organophosphorus pesticide residues is 0.05-0.1 ng·g-1 （S/N=3）. It is concluded that contents of 15 organophosphorus pesticide residues in hawthorn are determined. The established method can be used in the simultaneous determination of contents of organophosphorus pesticide residues.