慢性乙型肝炎患者肝组织低氧诱导因子-1α表达与肝脏微循环改变的关系

The relationship between the expression of hypoxia-induciable factor-1α and changes of hepatitis microcirculation in patients with chronic hepatitis B

目的探讨慢性乙型肝炎患者肝组织低氧诱导因子-1α的表达与肝脏微循环改变的关系。方法对120例慢性乙型肝炎患者肝组织进行观察，采用免疫组化方法观察低氧诱导因子-1α在肝组织中的表达变化，采用透射电镜观察肝脏微循环结构的改变情况。结果慢性乙型肝炎肝组织超微结构改变有肝窦腔狭窄、肝窦阻塞、肝窦扩张和肝窦毛细血管化几种主要形态，免疫组化观察低氧诱导因子-1α呈阳性或强阳性表达，与肝脏微循环障碍程度呈明显的正相关。结论慢性乙型肝炎肝脏微循环改变加重了肝细胞与血液循环之间的物质和氧的代谢障碍，肝细胞缺氧是肝脏病变的重要原因。

Objective To explore the relationship between the expression of Hypoxia-induciable factor-1α （HIF-1α） and changes of hepatitis microcirculation in patients with chronic hepatitis B （CHB）. Methods 120 patients with CHB were studied. The expression of HIF-1α was observed by immunohistochemistry and the morphotogic changes were observed with transmission eletron microscope. Results Stenosis, blockage,dilataltion,capillarization of hepatic sinusoids were the main morphotogic changes of liver. The expression of HIF-1α was positive or stronger positive. There is a positive correlation between the expression of HIF-1α and microcirculation disturbance of liver. Conclusion The disturbance of liver with CHB could exacerbate the dysbolism of nutrient substance and oxygen between hepatic ceils and blood circulation. Hypoxia of hepatic cells was an important reason of the liver＇s pathological changes.