四川部分高原地区小肠结肠炎耶尔森菌调查

Investigation on Yesinia Enterocolitica in Some Plateau Areas of Sichuan

目的为了解四川省高原地区喜马拉雅旱獭是否携带小肠结肠炎耶尔森菌及该菌是否和鼠疫耶尔森菌存在共生关系。方法 鼠疫常规监测中捕获的喜马拉雅旱獭,解剖时无菌操作取舌根和回盲肠内容物,置于准备好的10～15 ml改良PBS增菌培养管中,在4℃冰箱中增菌10～20 d后分离培养。结果在德格县采样100份,分离小肠结肠炎耶尔森2株,若尔盖县采样148份,分离小肠结肠炎耶尔森菌1株。结论 四川省高原地区喜马拉雅旱獭携带小肠结肠炎结肠炎耶尔森菌,该菌和鼠疫耶尔森菌可能存在共生关系。

Objective To understand whether the Himalayan marmots which lived in Sichuan Western Plateau carrying Yersinia enterocolitica,and investigate whether the symbiotic relationship exists between Yersinia enterocolitica and Yersinia pestis.Methods The Himalayan marmots in this study were captured from routine monitoring of plague,tongue basis and the contents in cecum were taken under aseptic operation,and be placed in the prepared 10-15ml modified PBS tubes.All the samples cultivated 10-20 days in the PBS under 4℃.Results Among 100 Marmota himalayana samples from Dege county,2 strains of Yersinia enterocolitica were detected,and 1 Yersinia enterocolitica strain was screened from 148 samples from Ruoergai County.Conclusion The Himalayan marmots living in Sichuan western plateau carry the Yersinia enterocolitica,and the symbiotic relationship possibly exists between Yersinia enterocolitica and Yersinia pestis.