拉萨与漳州地区战备消毒包储存时限的实验研究

Investigation of shelf life of sterile packages in Lhasa and Zhangzhou regions.

目的探讨战备消毒包在拉萨与漳州地区自然环境中的储存时限、影响因素及细菌来源。方法准备双层棉布手术敷料包、器械包、敷料器械混合包,预真空压力蒸汽灭菌后分别存放在自然环境战备仓库储存箱内,分别于灭菌后不同时限点随机取3种规格消毒包各3个进行细菌培养;同时对战备仓库空气、储存箱进行取样培养,并进行细菌同源性研究。结果灭菌消毒包储存时限拉萨地区为91 d,漳州地区为24 d;两地区战备消毒包、仓库空气和储存箱优势菌属为革兰阳性杆菌和球菌;消毒包内培养出的细菌与空气、储存箱分离出来的相同菌种不同菌株之间的16SrRNA-23SrRNA转录间隔区(interhaltranscribed spacer,ITS)序列相似性,在拉萨地区约为99%～100%,漳州地区约为98%～99%。结论拉萨地区灭菌消毒包储存时限长于漳州地区;不同地区战备消毒包的储存环境对其储存时限具有重要的影响;战备消毒包内细菌来源于仓库自然环境空气。

Objective To study the shelf life, influential faetors and origin of bacteria for sterile packages for war preparation stored up in Lhasa and Zhangzhou in the natural enviromnent. Methods Operation dressing packages ,apparatus packages as well adressing and apparatus mixed patkages with double - layer totton cloth were prepared and stored them in storage boxes in warehouses in a natural environment after vacuum steam sterilization. Three patkages of each kind were put in a storage boxe and samples were taken from the patkages and underwent bacterial culture at different time - intervals. Meanwhile, the batterial strains in tbe air of the warehouse and in the storage boxes were tullured and the bacterial isogeny was studied. Results The shelf life of sterile packages for war preparation was 91 d in Lhasa but 24d in Zhangzhou. The main bacleria tBund were gram - positive bacilli and gram - positive staphylococe in the two regions. In Lhasa, the 16SrRNA -23SrRNA ITS sequentes of batterial strains in sterile packages shared about 9%, - 100% （汉语摘要中是99% - 100% ）isogeny between the air bacterial strains in the warehouse and those in the storage boxes. but in Zhangzhou the isogeny was about 98% -99%. Conclusions The shelf life of sterile packages for war preparation is longer in Lhasa than in Zhangzhou. The storage environment is the most influential factor on shell life of sterile packages. The bacteria in slerile packages come from the air in the warehouse.