高糖对人肾小球系膜细胞p38MAPK／CREB通路与纤维化的影响

The effect of high glucose on p38MAPK/CREB pathway and fibronectin in human mesangial cells

目的探讨高糖环境下人肾小球系膜细胞中p38丝裂原活化蛋白激酶／cAMP反应元件结合蛋白（p38MAPK／CREB）通路的活性及细胞外基质成分纤维黏连蛋白（FN）的变化。方法以5．6mmol／L葡萄糖培养基培养肾小球系膜细胞为对照组，观察高糖（30mmol／L）培养12、24、48小时。肾小球系膜细胞p38MAPK／CREB活性和FNmRNA、FN的变化。结果与对照组比较，高糖组各时间p-p38MAPK、P—CREB的表达均上升，并随着时间的延长，其表达逐渐升高；FN的表达升高，并且随着时间的延长，其表达进一步升高。结论高糖环境下p38MAPK／CREB通路被激活，FN表达增加，p38MAPK／CREB通路参与了细胞外基质重构的过程。

Objective To investigate the activity of p38MAPK/CREB pathway and the change of fibronectin（FN） in human mesangial cell（HMCs） cultured under high glucose condition. Methods HMCs which were treated with 5.6 mmol/L glucose medium can be thinked as Normal control group, we can observe the change of the activity of p38MAPK, CREB, protein of FN, and mRNA of FN; Western Blot was used to assess the expression of protein of p-p38MAPK and p-CREB;Semi-quantitative RT-PCR was used to assess the expression of FNmRNA;Elisa was used to assess the expression of FNo Results Compare with the Normal control group,expression of p-p38MAPK and p-CREB were significantly enhanced and with the prolongation of time,the expression were higher and higher;expression of FN was also increased, and with the prolongation of time, the expression were higher and higher. Conclusions p38MAPK/CREB pathway was activitied under high glucose condition, and the expression of FN increase, so p38MAPK/CREB pathway may take part in the reconstitution of extracellular matrix（ECM）.