从大豆糖蜜发酵液中提取、纯化低聚糖

Oligosaccharide Extraction and Purification from Fermentative Broth of Soybean Molasses

为获得纯度较高的大豆低聚糖,研究大豆糖蜜发酵液去蛋白、脱色和脱盐等工艺。用4%HCL调节发酵液pH值为3,搅拌时间10 min,离心转速6000 r/min,蛋白去除率55.50%。采用AB-8大孔树脂,在pH 3,液体流速1.5BV/h,处理量4.5倍树脂床体积(BV)时,发酵液脱色效果最好,脱色率90%以上。当001×7和D301型离子交换树脂联用时脱盐效果较好。纯化获得的大豆低聚糖为白色透亮粉末,味微甜,总糖占固含物的94.00%,功能性成分占固形物的86.52%。

In order to obtain highly pure soybean oligosaccharides,the removing technology of protein,pigment and salts from fermentative broth of soybean molasses has been studied.The results showed that：with 4% HCL adjusting pH 3,6 000 r/min centrifugal speed and 10 min stirring,protein removal rate was 55.50%.The optimal decolorizing conditions of AB-8 macroporous resin were：pH 3,liquid velocity 1.5 BV/h and processing capacity 4.5 BV.Ion exchange resins 001×7 and D301 used together was the best in desalination.Purified soybean oligosaccharides was white translucent powder,taste sweet.The total sugar content and functional components content equal to 94.00% and 86.52% of the amount of solid content,respectively.