胎盘组织中HIF-1α的表达与血清sFlt-1，sEng水平在子痫前期的作用研究

Relationship of Hypoxia-inducible Factors-1α and Serum Soluble Endogln,Soluble fms-like Tyrosine Kinase Receptor-1 in Pathogenesis of Preeclampsia

目的探讨胎盘组织中缺氧诱导因子-1α（HIF—1α）的表达和血清中可溶性Endoglin（sEng）、可溶性血管内皮细胞生长因子受体-1（sFlt-1）水平在子痫前期发病机制中的作用。方法采用免疫组化SABC法检测20例正常晚期妊娠孕妇（对照组）和35例子痈前期患者（予痫前期组，其中轻度16例、重度19例）胎盘组织中HIF—1α的表达；采用酶联免疫吸附实验的方法检测其血清中sEng和sFh—1水平。结果①轻、重度子痈前期组中HIF-1α在合体滋养细胞的表达显著高于对照组（H=19．3，P〈0．05；H=23．1，P〈O．01）。②轻、重度子痫前期组血清sEng，sFlt-1浓度分别为（4．39±0．48）ng／m1，（5．61土0．66）ng／ml；（22．69±5．38）Pg／L，（30．15±5．43）vg／L，均显著高于对照组（1．71±0．27）ng／ml，（6．61±2．43）pg／L，差异具有统计学意义（t=21．25，P〈0．01;t=24．47，P〈0．01）；（t=11．96，P〈0．01；t=17．63,P〈0．01）。重度组高于轻度组0=6．17，P〈0．01；t=4．06，P〈0．01）。③子痫前期胎盘组织中HIF-1α表达与血清sEng，sFlt-1水平呈正相关（r=0．76，P〈0．01；r=0．87，P〈0．01）。结论HIF-1α和sEng，sFlt-1共同参与子痫前期的病理生理过程，并与病情严重程度有一定关系。

Objective To investigate the expression of hypoxia-inducible factors-1α in （HIF-1α） placenta and serum soluble endoglin,soluble fins-like tyrosine kinase receptor-1 in maternal serum from normal pregnant women and preeclampsia,in order to probe the possible mechanism of preeclampsia. Methods The expression of HIF-1α was detected by immunohis- tochemistry. The levels of sEng and sFh-1 in serum were detected by enzyme linked immunosorbent assay. Results （1）The expressions of HIF-1α in placental villous syncytiontrophoblast of preeclampsia group were significantly higher than that of control group （H = 19.3,P〈0.05 ; H = 23.1 ,P〈0. 01）. （2）The level of sEng,sFh-1 in serum of the the mild preeclampsia group （4. 39± 0. 48）ng/ml, （22.69±5.38） μg/L and the sever group （5.61±0. 66） ng/ml, （0. 15±5.43）μg/L were significantly higher than that of control group （1. 71±0. 27） ng/ml, （6. 61±2.43） μg/L; （t=21.25,P〈0. 01 ;t=24. 47,P〈 0. 01） and （t=11. 96,P〈0. 01 ;t= 17.63,P〈0. 01）. The sever group was much higher than the mild group （t=6. 17,P〈 0. 01 ;t= 4.06,P〈0. 01）. （3）There were significant correlation between he expressions of HIF-1α in placental and the level of sEng ,sFh-1 in serum of mild preeclampsia group and sever group （r= 0. 76 ,P〈0. 01 ;r=0. 87 ,P〈0. 01）. Conclusion HIF-1α,sEng and sFh-1 not only paticipated in pathogenic process of preeclampsia but also related to its degrees.