系统性红斑狼疮患者外周血中性粒细胞和单核细胞凋亡的研究

Study on Peripheral Blood Neutrophils and Mononuclear Cell Apoptosis with Systemic Lupus Erythematosus

目的比较系统性红斑狼疮（SLE）患者中性粒细胞及单核细胞．月亡与对照组差异，并探讨SLE患者中性粒细胞和单核细胞凋亡与某些相关血清因素的关系。方法对29例SLE患者和14例健康志愿者进行了研究。分离培养0，5，24h后的中性粒细胞和单核细胞凋亡检测用AnnexinV检测试剂盒争流式细胞仪检测。C3，C4和Ig等采用免疫散射比浊法。结果SLE患者中性粒细胞和单核细胞凋亡指数显著高于对照组，中性粒细胞培养5，24h凋亡率分别为（9．65±6．72）％和（68．36±9．51）％，与对照组比较P〈0．01，差异有统计学显著性意义；单核细胞培养24h凋亡率为（25．36±5．62）％，与对照组比较差异亦有统计学显著性意义（P〈0．01）。并且SLE患者血清补体与中性粒细胞和单核细胞的凋亡率之间存在一定相关性。结论SLE患者中性粒细胞和单核细胞凋亡加速，并且患者血清因素异常可能与细胞凋亡相关。

Objective To compare apoptosis of neutrophils and mononuclear cells in patients with systemic lupus erythe- matosus between the control group,assess the rate of apoptosis of neutrophils and mononuclear cell associated with certain serum factors. Methods 29 cases of SLE patients and 14 healthy volunteers were studied. To detect the neutrophils and mononuclear cell apoptosis by Annexin V detection kit and flow cytometry after cultured 0, 5h and 24h. C3,C4, Ig, etc. Serum factors were detected by immune nephelometry. Results After 5 and 24 hours cultured,the percentage of apop- totic neutrophils cell from patients with SLE were（9.65±6.72）% and （68.36±9.51）% ,significantly higher from control group. Mononuclear cells cultured for 24 hours apoptosis index was （25.36±5.62）%, compared with the control group difference was also significant. Serum complement and immunoglobulin may be related to neutrophils and mononuclear cell apoptosis rate. Conclusion Acceleration of apoptosis in vitro of neutrophils and mononuclear cell in SLE patients may be related to the abnormal serum factors.