伴生蕨类植物满江红提取物对水稻生长的影响

Effects of Companion Fern Azolla imbricata Extracts on Rice Growth

通过室内生物测定的方法研究满江红提取物对水稻种子萌发及幼苗生长的影响,并就提取物浓度与水稻生长相关指标间的关系进行回归分析。结果表明,与对照相比,250~4 000μg/mL的提取物对水稻种子萌发不产生显著影响;1 000μg/mL的醇提物和水提物分别对水稻幼苗根长和苗高产生显著促进作用,相对应的促进率为27.32%和9.15%;250~4 000μg/mL的醇提物和4 000μg/mL的水提物对水稻幼苗苗高,1 000~4 000μg/mL的水提物对水稻幼苗根长,2 000~4 000μg/mL的醇提物和4 000μg/mL的水提物对水稻幼苗单株鲜重产生显著抑制作用,最大抑制率分别为31.85%、14.28%、23.41%、16.49%和10.94%。拟合满江红醇提物浓度与水稻幼苗根长、苗高和单株鲜重的关系（根长y=-0.215x2＋1.541x＋5.133,p=0.001;苗高y=-0.035x2-0.062x＋5.574,P〈0.001;鲜重y=-0.685x2＋1.484x＋100.0,P〈0.005）以及水提物浓度与水稻幼苗根长、苗高和单株鲜重的关系（根长y=-0.005x2-0.276x＋7.012,P〈0.001;苗高y=-0.123x2＋0.760x＋4.490,P〈0.001;鲜重y=-1.742x2＋10.49x＋89.27,P〈0.005）均以二次曲线函数较佳。

Using the method of laboratory bioassay,the interference of the extracts from Azolla imbricata on the growth of rice was primarily studied.The results showed that the extracts with different concentrations had influences of different degree on the seed germination and seedling growth of rice.Extracts at concentrations of 250~4 000 μg/mL had no significant effects on rice seed germination;ethanol and aqueous extracts at concentration of 1 000 μg/mL could enhance the seedling root length and plant height significantlyby 27.32% and 9.15%,respectively;ethanol extracts at concentrations of 250~4 000 μg/mL and aqueous extracts at concentration of 4 000 μg/mL showed significant inhibition on rice seedling height,the same as aqueous extracts at concentration of 1 000~4 000 μg/mL on rice seedling root length;ethanol extracts at concentrations of 2 000~4 000 μg/mL and aqueous extracts at concentration of 4 000 μg/mL on seedling fresh weight per plant,the relative highest inhibition rates was 31.85%,14.28%,23.41%,16.49% and 10.94% respectively.Quadratic models were better for fitting the relationship between rice seedling root length,height,fresh weight per plant and ethahol extracts concentrations（root length： y=-0.215x2＋1.541x＋5.133,p=0.001;seedling height： y=-0.035x2-0.062x＋5.574,p〈0.001;fresh weight per plant： y=-0.685x2＋1.484x＋100.0,p〈0.005）,between seedling root length,height,fresh weight per plant and aqueous extracts concentrations（root length： y=-0.005x2-0.276x＋7.012,p〈0.001;seedling height： y=-0.123x2＋0.760x＋4.490,p〈0.001;fresh weight per plant： y=-1.742x2＋10.49x＋89.27,p〈0.005）.