无核荔枝凝集素基因的克隆与表达分析

Cloning and Expression Analysis of a Lectin Gene from Wuhe Litchi

应用PCR技术克隆了无核荔枝凝集素(LcLec)基因的部分cDNA(JF920723)和gDNA(JF920724)序列,序列分析表明,获得的cDNA序列含有一个468 bp的完整开放读码框结构,编码含155个氨基酸残基的多肽序列,该氨基酸残基序列与木菠萝家族的甘露糖结合凝集素同源。获得的gDNA序列含有3个内含子,长度分别为124、108和119 bp。荧光定量PCR结果表明,LcLec基因的表达量在无核荔枝果皮发育前期上升,之后下降至稳定水平;在采后果皮衰老阶段,随果皮褐变指数上升LcLec基因表达量上升。

Partial cDNA（JF920723）and gDNA（JF920724）sequences of a lectin gene（LcLec）were cloned from Wuhe litchi（Licthi chinensis Sonn.cv.Wuhe）using PCR.Sequence analysis showed that the cDNA sequence had an open reading frame（ORF）with 468 bp in length,encoding a polypeptide of 155 amino acids.The deduced amino acid sequence of LcLec had the typical conserved domain structure as mannose-binding lectin.The gDNA sequence contained three introns,the length of each intron was 124 bp,108 bp and 119 bp,respectively.RT-PCR analysis showed that the expression of LcLec in pericarp increased at early stage of fruit development and then decreased till fruit maturation.During the postharvest storage,the expression of LcLec in pericarp increased together with the percarp browning index.