Research on Algistatic Activities of Allelochemicals in Magnolia Grandiflora Leaves and GC-MS Analysis

广玉兰叶片化感物质的抑藻活性及GC-MS分析(英文)

[Objective]In order to investigate the antialgal activities and species of the allelochemicals in Magnolia grandiflora leaves.[Method]The antialgal substances against Microcystic aeruginosa from the extracts of Magnolia grandiflora leaves were separated,purified and identified based on the activity test,using silica gel column chromatography,gel column chromatography and GC-MS method and combining the algae inhibition experiment.[Result]The maximum inhibition rate of the extracts of Magnolia grandiflora leaves on Microcystic aeruginosa growth was 97.4% with the concentration of 8 g/L.A large amount of antialgal substances were found in the n-butanol extracts of Magnolia grandiflora and had very good inhibition effects on Microcystic aeruginosa,which were mainly small molecule substances such as alcohols,ketones and esters.[Conclusion]A new idea on developing new algal inhibitors had been provided.

[目的]研究广玉兰叶片化感物质的抑藻活性和种类。[方法]在活性测试的基础上利用硅胶柱层析、凝胶柱层析和GC-MS等方法,结合抑藻试验对广玉兰叶片提取物进行了铜绿微囊藻抑制活性物质的分离纯化和结构鉴定。[结果]广玉兰叶片浸取物在浓度8g/L的条件下对铜绿微囊藻生长的抑制效果最高可达97.4%;其正丁醇提取物中含有大量的抑藻活性物质,能够对铜绿微囊藻起到很好的抑制效果;这些具有抑藻活性的物质主要为小分子的醇类、酮类和酯类。[结论]为新型藻类抑制剂的开发提供了新思路。