维吾尔族子宫颈癌中HPV16变异体的初步研究

HPV16 Variants in Uighur's Cervical Cancer

为检测新疆维吾尔族宫颈癌中HPV16变异情况,并建立HPV16系统生成树。选用HPV16感染的维吾尔族宫颈癌40例,德国HPV16标准株做阳性对照,PCR扩增HPV16E6后纯化并双向测序;应用Lasergene、DNAstar、clustal 1.83、Mega 4.1软件对HPV16E6序列进行比对分析,并最终生成系统生成树。结果显示:在40例维吾尔族宫颈癌中发现HPV16变异位点4个:350nt(T→G),310nt(T→G),295nt(T→G),135nt(A→C);变异率最高位点为350nt(71%,20/28);相应氨基酸变化为L→V,F→L,D→E,K→T。通过系统生成树发现,本组样本全为欧洲变异体;HPV16E6 350位点变异与分化程度及年龄分组分别作比较(P>0.05),两者之间无统计学差异。由此可知,从系统生成树角度分析,维吾尔族宫颈癌中HPV16变异体为欧洲变异体;突变率最高位点为高危的350位点,其次为310、295、135位点,这些变异位点与维吾尔族宫颈癌的相关性有待进一步研究。

Objective to detected the variants of HPV16 and structured HPV 16 variants＇ phylogenetic tree of Uighur＇s cervical squamous cell careinoma（CSCC）. Methods.. selecting 40 cases of Uighur＇s CSCC, which had HPV16 infected; Using HPV16 standard strain as positive control and amplifieate HPV16 E6 genes,which were sequenced,blasted and analyzed by Lasergene, DNAstar. Finally,phylogenetic tree of HPV16 was drawed by using clustal 1.83,Mega4. 1 program; all the statistical dates were analyzied by SPSS17.0 software package. 4 variant sites of HPV16 were found in 40 cases of Uighur＇s CSCC： 350nt（T→G） ,310nt（T→G） ,295nt（T→G）, 135nt （A→C） ;the most frequently variant site was 350nt, （71 %,20/28） ;The variation of corresponding amino acids were L→V,F→L,D→E, K→T. Following by phylogenetic tree was structured, we found all of the cases in this group were E variant; Compared HPV 350 site＇s variant rate with age,differentiation degree,their relations were no statistical significance（P〉0. 05）. By the aspect of phylogenetic tree, the variant of HPV16 was E variant in Uighur＇s CSCC; the most frequently variant sites were 350 ,the follow sites were 310,295,135 sites. It need further research to identify if these variant sites and variant braeh were related with CSCC.