NPY、TSH-β、CaBP-28K基因在鸭繁殖期组织中的表达水平分析

The mRNA Expression Level of NPY,TSH-β and CaBP-28K Gene in Reproductive Periods of Duck

本研究对繁殖期高邮鸭神经肽Y(NPY)、促甲状腺激素β(TSH-β)、钙结合蛋白-D28K(CaBP-28K)基因在中枢神经组织和周围组织表达量进行了实时荧光定量分析。结果表明:NPY基因在下丘脑中表达量显著高于在垂体和在小肠中的表达量(P<0.05),在卵巢、心脏、肝脏、脾脏、肾脏、胰腺、子宫、胸肌、腿肌等9个部位呈痕量表达;TSH-β基因在垂体、小肠中表达量较高,显著高于其他组织(P<0.05);CaBP-28K基因在小肠表达量最多,其次是卵巢和肾脏,但均显著大于垂体中表达量(P<0.05),在其他测定部位呈痕量表达,就生殖轴而言,CaBP-28KmRNA的表达量表现为卵巢>垂体>下丘脑。本研究为了解高邮鸭繁殖期相关基因的表达特征和内分泌机制,指导高邮鸭选种选育提供了理论依据。

In the central nervous tissue and surrounding tissue,the mRNA expression level of NPY,TSH-β,CaBP-28K of Gaoyou Duck in reproductive period was analyzed by real-time fluorescent quantitative PCR.The result showed thatNPY gene expressed abundantly in hypothalamus which was very significant difference among other tissues（P0.05）,lessly in pituitary and intestines,tracely in ovary,heart,liver,spleen,kidney,pancreas,uterus,pectoral muscle and leg muscle.TSH-β was expressed abundantly in pituitary and intestines,which was significantly different to the other tissues（P0.05）,CaBP-28K was expressed mostly in intestine,and then ovary,kidney which was significantly different to pituitary（P0.05）,lessly in other tissues.The expression of CaBP-28K gene in HPG showed ovary pituitary hypothalamus.The findings of this study would contribute to further developing the expression feature and endocrine mechanism,and directing the Gaoyou duck breeding.