摘要
本研究对繁殖期高邮鸭神经肽Y(NPY)、促甲状腺激素β(TSH-β)、钙结合蛋白-D28K(CaBP-28K)基因在中枢神经组织和周围组织表达量进行了实时荧光定量分析。结果表明:NPY基因在下丘脑中表达量显著高于在垂体和在小肠中的表达量(P<0.05),在卵巢、心脏、肝脏、脾脏、肾脏、胰腺、子宫、胸肌、腿肌等9个部位呈痕量表达;TSH-β基因在垂体、小肠中表达量较高,显著高于其他组织(P<0.05);CaBP-28K基因在小肠表达量最多,其次是卵巢和肾脏,但均显著大于垂体中表达量(P<0.05),在其他测定部位呈痕量表达,就生殖轴而言,CaBP-28KmRNA的表达量表现为卵巢>垂体>下丘脑。本研究为了解高邮鸭繁殖期相关基因的表达特征和内分泌机制,指导高邮鸭选种选育提供了理论依据。
In the central nervous tissue and surrounding tissue,the mRNA expression level of NPY,TSH-β,CaBP-28K of Gaoyou Duck in reproductive period was analyzed by real-time fluorescent quantitative PCR.The result showed thatNPY gene expressed abundantly in hypothalamus which was very significant difference among other tissues(P0.05),lessly in pituitary and intestines,tracely in ovary,heart,liver,spleen,kidney,pancreas,uterus,pectoral muscle and leg muscle.TSH-β was expressed abundantly in pituitary and intestines,which was significantly different to the other tissues(P0.05),CaBP-28K was expressed mostly in intestine,and then ovary,kidney which was significantly different to pituitary(P0.05),lessly in other tissues.The expression of CaBP-28K gene in HPG showed ovary pituitary hypothalamus.The findings of this study would contribute to further developing the expression feature and endocrine mechanism,and directing the Gaoyou duck breeding.
出处
《中国畜牧杂志》
CAS
北大核心
2011年第17期6-9,共4页
Chinese Journal of Animal Science
基金
国家科技支撑计划项目(2008BADB2B08)
江苏省科技服务平台(BM2008170)