双氢青蒿素、青蒿琥酯和蒿甲醚连续给药及伍用治疗小鼠血吸虫病效果观察

Effects of dihydroartemisinin,artemether,and artesunate administered orally in multiple doses or in combination to treat mice with schistosomiasis

目的观察双氢青蒿素、青蒿琥酯和蒿甲醚连续给药或伍用治疗小鼠血吸虫病的效果,为日本血吸虫病病原学治疗提供药物配伍实验依据。方法采用腹部贴片感染法,每鼠感染日本血吸虫尾蚴40±1条,分别于感染后6~8 d（童虫期）和34~36 d（成虫期）,以300 mg/kg双氢青蒿素、青蒿琥酯和蒿甲醚连续给药及3种药物等剂量配伍给药,在感染后50 d解剖小鼠,收集成虫,计算减虫率和减雌率。结果在感染后6~8 d,双氢青蒿素、青蒿琥酯、蒿甲醚连续3 d单独给药和3种药物等剂量配伍给药,小鼠减虫率为79.5%~86.0%;减雌率为79.4%~86.7%,差异均无统计学意义（P均〉0.05）;在感染后34~36 d给药,减虫率为73.8%~75.8%,减雌率为88.7%~93.1%,差异无统计学意义（P均〉0.05）。结论双氢青蒿素、青蒿琥酯和蒿甲醚连续给药及伍用治疗小鼠血吸虫病效果无显著差异。

Objective To investigate the effects of dihydroartemisinin,artesunate,and artemether administered orally in multiple doses or in combination to treat mice with schistosomiasis in order to provide an experimental basis for treatment of the causes of schistosomiasis japonica.Methods Mice were infected with 40±1 Schistosoma japonicum cercariae via the abdomen and treated with dihydroartemisinin,artesunate,and artemether at multiple doses or in combination（all doses were 300 mg/kg） on days 6-8 or 34-36 post-infection.Mice were sacrificed on day 50 post-infection,the adult worms were recovered,and the total and female worm reduction rates were calculated.Results Administration of dihydroartemisinin,artesunate,or artemether for 3 successive days reduced the total worm burden by 79.5%-86.0% and the female worm burden by 79.4%-86.7% depending on the treatment protocol followed on days 6-8 post-infection,though differences in the total and female worm burden were not statistically significant（all P values 0.05）.When the same treatment was given on days 34-36 post-infection,the total worm burden decreased 73.8%-75.8% and the female worm burden decreased 88.7%-93.1%,though differences in the total and female worm burden were not statistically significant（all P values 0.05）.Conclusion Administration of dihydroartemisinin,artesunate,and artemether in multiple doses or in combination affected both juvenile and adult S.japonicum,but there was no obvious difference in the effectiveness of the two forms of administration.