远志中的三萜皂苷和寡糖多酯类成分(英文)

Triterpenoid Saponins and Oligosaccharides from the Roots of Polygala tenuifolia Willd

目的:研究远志的抗老年痴呆活性成分。方法:采用活性追踪的方法,利用硅胶柱层析、反相中压色谱、制备高效液相等多种色谱技术分离单体化合物;并利用多种光谱技术确定化合物的结构。结果:从远志的根中分离得到了5个三萜皂苷类化合物Onjisaponin Wg(1),Fg(2),Ng(3),R(4)和O(5)及6个寡糖多酯类化合物tenuifolioses A(6),B(7),C(8),I(9),K(10),和G(11)。结论:化合物1为新化合物,化合物2-5首次报道其核磁数据;化合物6和7显示了PC12细胞的保护作用;化合物7和8可增强大鼠齿状回突触的传递作用,表明具有改善大鼠学习记忆的活性。

AIM： To study the chemical constituents and bioactivities of the roots of Polygala tenuifolia Willd.. METHODS： Bioactivity-guide was applied to study the chemical constituents of P. tenuifolia, and the chemical structures were determined by application of spectroal data （NMR, MS）. RESULTS： One new, four known triterpenoid saponins and six oligosaccharides were obtained and their structures were identified as 3-O-β-D-glucopyranosyl-presenegenin 28-O-α-L-arabinopyranosyl-（1→3）-β-D-xylopyrano- syl-（1→4）-[β-D-apiofuranosyl-（1→3）]-a-L-rhamnopyranosyl-（1→2）-β-O-3, 4, 5-trimethoxycinnamoyl] -β-D-fucopyranosyl ester （onjisaponin Wg, 1）, onjisaponins Fg （2）, Ng （3）, R （4）, and O （5）, tenuifolioses A （6）, B （7）, C（8）, I （9）, K （10）, and G （11）. CONCLUSION： Compound 1 is a new saponin. Compounds 6 and 7 showed neuroprotective activity against glumate and serum deficiency at the concentration of 1 × 10^-5 mol·L^-1. Compounds 7 and 8 potentiated basal synaptic transmission in the dentate gyrus of anesthetized rats. The NMR data of 2-5 were reported for the first time.