小叶女贞对免疫功能损伤小鼠B淋巴细胞和NK细胞的影响

A study of the effect of ligustrum ouihoui carr （LQC）on the immune function of Blymphocytes and NK lymphocytes in mouse with impaired immune function

目的探讨小叶女贞对免疫功能损伤小鼠B淋巴细胞、NK细胞免疫功能的影响。方法以环磷酰胺致免疫功能损伤小鼠为研究对象，将BALB／C实验小鼠60只按随机数字表法随机分为5组，每组12只，分别为生理盐水（NS）对照组、环磷酰胺（Cy）对照组、Cy＋小叶女贞水煎液小剂量组（小叶女贞小剂量组）、Cy＋小叶女贞水煎液大剂量组（小叶女贞大剂量组）、Cy＋女贞子水煎液组（女贞子组）。NS对照组给予生理盐水0．2ml／d皮下注射10d，其余各组给予环磷酰胺0．8ml／d皮下注射（20mg／kg·d^-1）10d，建立免疫功能损伤小鼠模型。自第11天起各组分别给予生理盐水、环磷酰胺、小叶女贞水煎液小剂量、小叶女贞水煎液大剂量、女贞子水煎液共7天处死。用流式细胞仪检测实验鼠外周血B淋巴细胞（CD3^-CD19^＋）和NK细胞（CD3^-CDl6^＋CD56^＋）的百分率。结果CD3^-CDl9’、CD3^-CDl6^＋CD56^＋细胞小叶女贞小剂量组[分别为（11．54±0．98）、（12．46±0．08）]，明显低于小叶女贞大剂量组[分别为（20．44±1．78）、（19．12±1．70）]、女贞子组[分别为（19．90±1．42）、（20．17±1．66）]，明显高于环磷酰胺组[分别为（4．53±1．70）、（5．03±1．22）]，差异均有统计学意义（P均〈0．01）；与NS对照组[分别为（11．84±0．99）、（12．90±0．28）]比较无明显改变（P〉O．05）。结论小叶女贞能提高小鼠B淋巴细胞和NK细胞细胞的数量，使小鼠受损伤的免疫功能得到改善，对免疫功能的调节作用与剂量成正相关。

Objective To explore the effect and mechanism of LQC on the immune function of Blymphocytes and NK lymphocytes in mouse with impaired immune function. Methods Mice with impaired immune function led by Cyclophosphamide （Cy）were used as experimental animals, and divided into five groups randomly, twelve mice in every group, which is NS control group, Cy control group, Cy^＋ LQC minor and major dose group, and Cy^＋LQC decoction group. NS control group was given NS 0.2 ml/d subcutaneously 10 d, and the rest groups were given Cy 0.8 ml/d subcutaneously （20 mg/kg· d^-1） 10d, to establish a mouse model of immune dysfunction. Since the llth day each group was given NS, Cy, LQC minor dose, major dose and fructus ligustri lucidi decoctoin. Mice of each group were killed after 7 days. The percentage of B lymphocytes （CD3^- CD19^＋ ） and NK lymphocytes （CD3 ^-CD16^＋CD56^＋ ） in the peripheral blood of the experimental mice were detected by flow cytometer. Results In comparison with LQC major dose group [ （20.44±1.78） and （ 19.12 ±1.70） ], fi＇uctus ligustri lucidi group [ （ 19.90 ±1.42） and （20.17± 1.66） ], CD3^-CD19^＋ and CD3^- CD16^＋CD56^＋ cells of LQC minor dose group[ （11.54±0.98） and （12.46±0.08）]were decreased significantly （P〈0.01）, which were increased significantly compared with Cy group [ （4.53 ± 1.70） and （5.03 ± 1.22） ] （P〈0.01）, but they had no significant difference with NS [ （11.84±0.99） and （12.90±0.28） ] （P〉0.05）. In comparison with Cy group and NS group, CD3^-CD19^＋ and CD3^-CD16^＋CD56^＋ cells of LQC major dose group were increased significantly （P〈0.01）, which had no significant difference with fructus ligustri lucidi group （P〉0.05）. Conclusion The immune functions of the mice with impaired immune function were improvedby LQC, it also could increase the quantity ofCD3^-CD19^＋ cell and CD3^-CD16^＋CD56^＋ cell The dose of LQC was positively correlated with the modulation effect of LQC on the immune function.