木瓜蛋白酶及胰蛋白酶所致大鼠肺气肿模型的差异及对比

Papain induced by trypsin and rat emphysema and comparison model

目的研究木瓜蛋白酶及胰蛋白酶所致大鼠肺气肿模型的差异。方法采用猪胰蛋白酶和木瓜蛋白酶气管内滴注的方法，将体质量170～220 g的60只SPF级SD大鼠随机分为正常对照组（A组）,猪胰蛋白酶组（B组），木瓜蛋白酶组（C组），各20只，B组、C组气管内分别滴入猪胰蛋白酶1 U/g、5％木瓜蛋白酶0.001 ml/kg造模，A组气管内滴入等量生理盐水，第90天处死大鼠，将大鼠肺组织在10％甲醛中固定24 h以上，取右下肺组织石蜡包埋切片，行HE染色，观察各组大鼠肺组织病理变化，行免疫组织化学染色，以平均光密度值反映血管内皮生长因子的表达，比较两组试剂的平均用量，平均费用，保存方法的差异。结果各组大鼠肺组织病理学改变示B组、C组大鼠肺泡结构紊乱，部分肺泡壁断裂，肺泡腔扩大，对照组肺泡结构未见明显变化。B组、C组平均肺泡面积，单位面积肺泡数，平均肺泡间隔差异无统计学意义。各组大鼠肺组织中血管内皮生长因子-2的表达B组、C组光密度值较A组明显增高，B组、C组之间差异无统计学意义。在大鼠体质量无明显差异的情况下，在试剂用量，试剂费用，保存方法方面，木瓜蛋白酶优于猪胰蛋白酶。结论木瓜蛋白酶及胰蛋白酶构建大鼠肺气肿模型效果无明显差异，在试剂用量，试剂费用，保存方法方面，木瓜蛋白酶优于猪胰蛋白酶。

Objective To study by trypsin and papain rats emphysema model. Methods Using pig trypsin and papain endotracheal drip, will 170-220 gram weight SPF 60 SD rats were randomly divided into normal control group （group A）, pig trypsin group （group B）, papain group （group C）, 20 in each group. Group B, C endotracheal drip respectively 1U/g, pig trypsin papain 5% papain 0.001 ml · kg-1 ,made group A drip endotracheal releases saline, 90 days, will be executed rat rat lung tissue fixed in 10% formalin for more than 24 hours, right lung tissue, paraffin slice observation group, wash. Dyeing rats,lung tissue pathology changes in immunohistochemical study, the average density of light reflects the expression, compare two VEGF reagent, the average consumption group, the average cost method.Results Each group rat lung tissue pathology change group B, C rats, the alveolar structure disorder,part of alveolar walls, the alveolar rupture cavity expansion, the alveolar structure without obvious changes. Group B, C, average per unit area of alveolar alveolar, average alveolar interval nonsignificant between-group difference. Each rat lung tissue VEGF-2 expression B, C group is A group of light density value significantly higher, B, C without difference between groups. In the rat weight no obvious difference in the circumstances, reagent consumption, reagent cost, preserving method, papain was better than pig trypsin. Conclusions Papain trypsin and constructing rats emphysema paients model, reagent consumption, reagent cost, preserving method, papain was better than pig trypsin.