双路频分复用荧光共焦显微探测技术研究

Two-Channel Frequency Division Multiplexed Fluorescence Confocal Microscopy

报道了结合频分复用技术来提高荧光共焦生物显微探测系统探测能力的实现原理和方法。通过对双路激发光信号的载频调制,将激发光聚焦到生物样品上产生荧光信号,再通过傅里叶变换、滤波和解调制过程,最后将两路荧光信号强度随时间变化曲线进行还原。实验搭建了紫外波段激励光源的双频复用荧光共焦显微成像系统,并成功探测到了鼠神经海马细胞样品发出的双点荧光信号。频分复用共焦显微成像系统能够多通道、实时、快速地探测生物细胞荧光信号,并具有较高的时间和空间分辨率。

We present a setup of confocal fluorescence microscopy system combined with frequency division multiplexing so that the detection ability of the system is enhanced. In the proposed frequency division multiplexed confocal fluorescence microscopy （FDMCFM） system, fluorescence is excited on the sample （rat neural cell） by two beams of light modulated individually in two arms of the system. Fourier transform, filtration and demodulation are then carried out to restore the fluorescence intensity signal. The FDMCFM system is experimentally conducted, through which the target cells are detected successfully. The experimental results show that the FDMCFM system is convenient for multiple-channels and real-time detection of biological cells＇ fluorescence signal. Moreover, it maintains the high spatial and temporal resolution of confocal microscopy.