地塞米松预处理在四氧嘧啶诱导1型糖尿病兔模型中的价值

Effect of dexamethasone pretreatment on alloxan induced diabetic rabbit model

目的 探讨地塞米松预处理在四氧嘧啶诱导1型糖尿病兔模型中的价值。方法按是否给予地塞米松预处理和四氧嘧啶给药次数将新西兰免分为四组，采用四氧嘧啶静脉注射诱导糖尿病模型，对病死率及成模率等进行比较。结果地基米松预处理组成模率为66．67％（1次给药法），83．33％（2次给药法）；无预处理组为16．67％（1次给药法），41．67％（2次给药法）。四组中地塞米松预处理2次给药法成模率最高（83．33％）。地塞米松预处理组病死率为25．00％（1次给药法），0．00％（2次给药法）；无预处理组为83．33％（1次给药法），41．67％（2次给药法）。四组中地塞米松预处理2次给药法病死率最低（0．00％）。结论地塞米松预处理，尤其是地塞米松预处理2次给药法可显著提高四氧嘧啶诱导糖尿病兔模型的成模率，明显降低病死率，缩短建模周期，是构建糖尿病兔模型的较理想方法，值得推广应用。

Objective To investigate the effect of dexamethasone pretreatment on alloxan induced diabetic rabbit model. Methods Male New Zealand rabbits were divided into four groups according to whether being pretreated with dexamethasone and administration times of alloxan. Fatality and success rate between the groups were compared. Results The success rate in dexamethasone pretreated group was 66.67% （once administration） and 83.33% （twice administration）, that of unpretreated group was 16.67% （once administration） and 41.67% （twice administration）. In the whole animals dexamethasone pretreated group with twice administration show the highest success rate. The fatality in dexametbasone pretreated group was 25.00% （ once administration） and 0.00% （ twice administration）, that of un - pretreated group was 83.33% （once administration） and 41.67% （twiceadministration）. In the whole animals dexamethasone pretreated group with twice administration show the lowest fatality. Conclusions Dexamethasone pretreatment especially combined with alloxan twice administration could enhance the success rate of diabetic rabbit induced by alloxan. Moreover, the fatality was decreased significantly and induction period was shortened. Therefore, it is the ideal strategy to induce diabetic rabbit model.