基质金属蛋白酶2和基质金属蛋白酶9、血管内皮生长因子在非小细胞肺癌中的表达及临床意义

Expression and clinic pathological features of MMP 2, MMP 9 and VEGF in non - small - cell lung carcinoma

目的 观察非小细胞肺癌（NSCLC）标本中基质金属蛋白酶2（MMP2）和MMP9及血管内皮生长因子（VEGF）的表达，以探讨其在NSCLC中表达的意义，为临床的诊断、治疗和预后的判断提供参考。方法用sP免疫组化技术检测MMP2、MMP9、VEGF在77例肺癌组织中的表达。结果肺癌组织中MMP2、MMP9主要表达于肿瘤细胞的胞浆，在癌旁交界区组织也有表达，肿瘤组织的过表达率显著高于交界区（P〈0．05），MMP2、VEGF在肿瘤组织的过表达率与淋巴结转移状态、临床分期与原发肿瘤大小有关（P〈0．05）。结论MMP2、MMP9、VEGF都参与了非小细胞癌发生、发展过程，MMP2、VEGF在其中可能起到协同促进作用，MMP2、VEGF联合分析可能更有助于评估NSCLC患者的预后。

Objective To investigate the expression and clinic pathological features of MMP2, MMP9 and VEGF in non - small - cell lung carcinoma, and provide reference for diagnosis, treatment and prognosis predictment of the disease. Methods MMP 2 and MMP 9, VEGF expressions were detected in 77 cases of non - small - cell lung carcinoma tissues and their adjacent tissues and 24 cases of normal lung tissues by steptavidinperoxdase immunohistochemical technique. Results The immunoreactivities of MMP 2 and MMP 9, VEGF were mainly shown in cytoplasma of tumor cells. The overexpressions of MMP 2 and MMP 9, VEGF in carcinoma tissues were significantly higher than in adjacent tissues（ P 〈 0. 05）. The overexpression of MMP 2 and VEGF in the cancer tissues were related to clinical stages, lymph node metastasis status and the size of primary cancer （ P 〈 0.05）. There was a trend that the survival rate of these patients with overexpression of MMP 2 and MMP 9, VEGF was lower than that with non - overexpression, but there was no statistical significance. The survival rate of the group with both MMP 2 and MMP 9,VEGF overexpressions was lower than with non - overexpression （ P 〈 0.05 ）. Conclusions MMP 2, MMP 9 and VEGF all take part in the progress of non - small - cell lung carcinoma. We postu- late that MMP 2 can cooperate with VEGF in the processes. To investigate the over - expressions of both MMP 2 and MMP 9 in non - small - cell lung carcinoma tissues may be more helpful for estimating the prognosis.