扁穗冰草转录因子基因的克隆和表达特性的分析

Cloning and expression analysis of transcription factor from Agropyron cristatum

根据禾本科转录因子cbf,dreb,myb基因已经报道的核酸序列保守区设计引物,使用RT-PCR技术首次在扁穗冰草(Agropyron cristatum)中克隆3个转录因子部分片段,分别将其命名为ACcbf,ACdreb,ACmyb。利用半定量RT-PCR技术研究这3个基因在冷胁迫及干旱胁迫下的表达趋势。结果表明,ACcbf基因对温度敏感,同时对干旱胁迫有一定程度的响应;ACdreb基因对冷胁迫和干旱胁迫都较为敏感;ACmyb基因则对干旱胁迫敏感,而对冷胁迫不敏感。

Primers were designed based on the conserved region of cbf, derb, myb in Gramineae plants. RT-PCR was used to clone transcription fragments from Agropyron cristatum leaf and 3 fragments of 548 bp, 862 bp, 319 bp were firstly obtained and named as ACcbf,ACdreb,ACmyb, respectively. Semi-quantitative RT-PCR was conducted to test the expression mode of the three genes under cold and drought stress. The result of this study showed ACchf was sensitive to cold stress, and more or less, responsed to drought, and ACdreb was sensitive to both drought and cold, and ACmyb was sensitive to drought, but insensitive to cold.