酶联免疫吸附试验两种方法检测梅毒螺旋体抗体结果比较

Comparison on results of measuring anti-syphilis antibody by one-step and two-step ELISA

目的比较梅毒螺旋体-酶联免疫吸附试验(TP-ELISA)一步法和二步法检测梅毒螺旋体抗体(抗-TP)的结果,研究钩状效应对TP-ELISA一步法检测抗-TP结果的影响,评价TP-ELISA二步法检测抗-TP的应用价值。方法分别用梅毒螺旋体明胶颗粒凝集试验(TPPA)、TP-ELISA一步法和二步法检测3 600份住院患者血清,并用TP-ELISA一步法(稀释法)检测怀疑存在钩状效应的血清标本12份。结果 TPPA、TP-ELISA二步法分别检测出91份抗-TP阳性,结果一致;TP-ELISA一步法检测出79份抗-TP阳性,12份TP-ELISA一步法检测结果阴性怀疑存在钩状效应的血清标本进行1∶5、1∶10、1∶40、1∶80倍稀释后,再采用TP-ELISA一步法进行检测,分别有12份、12份、8份、4份结果转为阳性。结论采用TP-ELISA二步法或TP-ELISA一步法同时对标本进行一定倍数稀释后检测,可以克服钩状效应,提高检测的敏感度。

Objective To compare the results of measuring anti-syphilis antibody by one-step and two-step ELISA,to investigate the influence of hook effect in measuring ant^syphilis antibody and to evaluate the advantages of two-step ELISA. Methods 3 600 samples of inpatients＇ sera were tested by TPPA,one-step TP-ELISA,two step TP-ELISA separately. 12 patientsr sera with suspected hook effect were measured with one-step ELISA（diluted）. Results 91 cases were diagnosed positive by TPPA and two-step ELISA,79 cases were diagnosed positive by one-step ELISA. After 1 ： 5,1 ： 10,1 ： 40,1 ： 80 dilution,12 cases of syphilis were detected again by one-step ELISA and 12, 12,8,4 cases turned to positive respectively. Conclusion The hook effect can be eliminated by two-step ELISA or one-step ELISA after diluting all samples and the detection sensitivity can be increased.