前列腺腺癌中DD3(Pca3)基因的表达及其意义

Expression of DD3(Pca3) RNA and Its Significance in Prostatic Carcinoma

目的评价DD3(Pca3)检测在前列腺腺癌活检标本病理诊断中的应用价值。方法观察DD3(Pca3)基因在196例前列腺腺癌和相关疾病前列腺标本中的原位杂交表达情况。结果在149例前列腺腺癌病例中,DD3(Pca3)染色阳性率为94.6%,染色情况与Gleason评分、患病年龄、血清总PSA值的关系无统计学意义。52例癌周有前列腺上皮内肿瘤(PIN)4,7例DD3(Pca3)阳性;149例腺癌癌巢周18例DD3(Pca3)阳性。在42例良性前列腺增生(BPH)中1例DD3(Pca3)阳性(2.38%)。结论 DD3(Pca3)是诊断前列腺腺癌有价值的阳性标记物。

Objective To evaluate the specificity and sensitivity of DD3 （Pea3） in detection of prostatic adenocarcinomas. Methods The expression of DD3 （ Pca3 ） was detected by in situ hybridization in 196 cases of prostatic adenocareinomas,42 cases of benign prostatic hyperplasia and 5 cases of atypical small acinar proliferation. Results DD3 （Pca3）RNA was positive in 94.6% cases of prostatic adenocarcinomas, regardless of Gleason score, age and serum PSA. However, DD3 （ Pca3 ） was also positive in high grade PIN that surrounded adeno- carcinomas （n = 52） ,47cases showed positive expression. In 149 cases of prostatic carcinoma, some normal-appearing glands situated in the immediate vicinity of cancer glands showed Pca3 expression. In 42 cases of BPH, lcases （2.38%） showed positive expression. Conclusion DD3 （Pca3）is a positive marker for diagnosis of prostatic adenocarcinomas,which has high sensitivity and specificity.