司帕沙星与对苯醌荷移反应的研究

Study on the Charge-transfer Reaction of Sparfloxcin with p-Benzoquinone

主要研究在硼砂缓冲溶液中，司帕沙星与对苯醌发生反应，生成稳定的1：1的络合物，其最大吸收波长为620．0nm，线性范围1～12μg／mL，表观摩尔吸光系数ε=1．99×10^4L／（mol·cm）。本方法所测定的药物制剂含量与文献方法相吻合，回收率（质量分数）在99．25％～100．5％，相对标准偏差为2．30％。

A new spectrophotometric method was developed for the determination of sparfloxacin based on a reaction of charge transfer with p-benzoquinone in borax buffer solution. The composition of complex of sparfloxcin with p-Benzoquinone was 1：1. The complex showed maximum absorption was at 620.0 nm. The apparent molar absorptivity was 1.99×10^4L/（mol·cm）. The calibration curves was linear in the concentration range of 1～12μg/mL. The recovery was 99.25%-100.5%. The method gave a RSD of 2.30% （n=5）. This method was found to be simple, quick and accurate for determination of sparfloxacin in tablets.