宜城市花生枯萎病病原的鉴定

Identification of Pathogen Causing Wilt Disease of Peanut in Yicheng City

对湖北宜城花生枯萎病的病原进行了生物学和分子鉴定,结果显示,6月份的病原主要是真菌,41份样品中,分离的真菌样品有38份,其中23份样品是黑曲霉菌。通过PSA培养基上真菌形态观察、真菌核糖体间区ITS区段的PCR扩增和序列分析,明确6月份花生枯萎病的病原分别是黑曲霉菌、立枯丝核菌和镰刀菌;8月份的病原主要为细菌,10份样品中,9份分离得到茄科雷尔氏菌(青枯病菌),通过细菌的回接试验,部分植株产生典型青枯、萎蔫症状,明确8月份的病原是青枯病菌。

The pathogen of peanut wilt disease in Yicheng city,Hubei province was identified by bioassay and molecular identification.The results indicated that 38 samples in 41 samples isolated in June were fungi,and among them 23 samples were Aspergillus niger.By PCR amplification and sequence analysis of ribosomal RNA ITS regions,the pathogen causing wilt disease of peanut in June were identified as A.niger,Rizoctonia solani and Fusarium sp.In August,the main pathogen was bacteria.9 samples were identified as Ralstonia solanacerum out of the 10 samples by typical morphological character of bacteria and symptom of peanut seedling after bacterial inoculation.