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辣椒叶片总RNA快速提取 被引量:1

Rapid Extraction of Total RNA from Capsicum annuum Leaves
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摘要 采用改良的Trizol法对辣椒(Capsicum annuum)叶片的总RNA进行了提取,利用琼脂糖凝胶电泳、紫外分光光度法、RT-PCR进行RNA纯度、完整性检测。结果表明,Trizol法提取可获得较高质量的辣椒叶片总RNA,能满足后续的研究需要。 Trizol extraction method was modified to extract total RNA from Capsicum annuum leaves.The result of agar gel electrophoresis,ultraviolet ray photometer and RT-PCR showed that the total RNA obtained by modified Trizol method was of high quality,and suitable for downstream applications.
出处 《湖北农业科学》 北大核心 2011年第17期3630-3631,共2页 Hubei Agricultural Sciences
基金 贵州省科学技术基金([2009]2104) 遵义市科技计划基金([2009]22) 贵州省教育厅自然科学研究项目(2010087)
关键词 辣椒(Capsicum annuum) 总RNA 改良Trizol法 提取 Capsicum annuum total RNA modified Trizol method extraction
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