地丁草组织培养及无性系建立的研究

Tissue culture and establishment of clone of Corydalis bungeana

目的：为满足人们栽培对种苗的需要，并保护野生资源。方法：以地丁草不同外植体为材料，进行了愈伤组织诱导与分化、不定芽生根和试管苗生根继代培养研究，建立起地丁草的无性系。结果：MS＋NH4H2PO4 20mg·L-1＋BA0．2mg·L-1＋IBA0．1mg·L-1＋NAA0．8～1．0mg·L-1是愈伤组织继代扩增培养的理想培养基；MS＋AgN030．5mg·L-1＋BA0．8mg·L-1＋NAA0．1mg·L-1是愈伤组织继代扩增培养的愈伤组织分化理想培养基。MS＋AgNO。0．5mg·L-1＋BA0．8mg·L-1＋NAA0．1mg·L-1是愈伤组织分化理想培养基。1／2MS＋IAA0．2mg·L-1-0．4mg·L-1是试管苗生根培养的理想培养基。1／2MS＋IAA0．3mg·L-1是试管苗生根继代培养的理想培养基。移植到草地上的试管苗保持了野生地丁草的所有生物性，当年鲜草产量增加32％。结论：建立起地丁草的无性系。

Objective ： To meet the need for cultivation of germchits and protect wild resources. Methods ： Dif- ferent explants from Corydalis bungeana were cultured in vitro to investigate callus inducement and differentia- tion, formation of adventitious roots,multiplication of adventitious roots and the clones of Corydalis bungeana was established. Results： The ideal medium for callus multiplication was MS＋NH4H2PO4 20mg·L-1＋BA0.2mg·L-1＋IBA0.1mg·L-1＋NAA0.8～1.0mg·L-1;the medium of MS＋AgN030.5mg·L-1＋BA0.8mg·L-1＋NAA0.1mg·L-1 was suitable for callus differentiation; 1/2MS＋IAA0.2mg·L-1-0.4mg·L-1 was the best rooting medium for the tube seedlings, and the multiplication of adventitious root ideal medium was 1/2MS ＋ IAA0.3mg·L-1. The tube seedlings retained all biological characteristics of the escape Corydalis bungeana af- ter transplanted to grassplot. The year yield of fresh grass was increased by 32%. Conclusion：The clone of Corydalis bungeana is established.