荧光分析法检测金藻对Zn^(2+)的利用

Application of Zn^(2+) to Detect Isochrysis Galbana by Fluorimetry

研究球等鞭金藻3011(Isochrysis Galbana 3011)对Zn2+的利用。采用FDA-PI双色荧光分析法对处理藻液进行流式细胞技术检测和荧光显微镜镜检,评价4 h和24 h的细胞活性,通过单因素对比试验设计,试验数据使用spss17.0统计软件进行方差分析。结果在活性浓度作用域中,未发现致毒现象,藻细胞长势良好,Zn2+处理Isochrysis Galbana 30114 h、24 h时,FDA-PI荧光检测显示Zn2+浓度为80μg/L时流式检测结果与对照组比较具统计学意义,藻细胞膜的完整性几乎没有受到影响,而FDA荧光检测则表明藻细胞的脂酶活性受到刺激。这一趋势在处理24 h时依然存在,并获得最大活度。研究表明80μg/L是Zn2+利用的最佳浓度域,说明Zn2+的摄入加速了细胞膜上磷脂代谢。

To study the application of Zn2＋ on Isochrysis Galbana 3011,the processed microalgae were detected by flow cytometry and fluorescence microscope by FDA-PI double fluorescent dyes.After 4 h and 24 h zinc exposure,cell activity was evaluated,through single-factor comparison experiment design and the result data was analyzed by variance analysis using statistical software of spss 17.0.The results showed that cells grew well,the toxicity was not found within the scope of activity concentration.There was significantly difference with control when Isochrysis Galbana 3011were treated with FDA-PI fluorescence detection by 80 μg/L Zn2＋ in the test.There was no significant effect on the membrane integrality in all concentration treatments,while modest stimulation of esterase activity was detectable by FDA staining.This trend still existed when cells were treated with 24 h.And maximum activity was gained.Optimal concentration of domain was 80 μg/L,the intake of Zn2＋ accelerated the metabolism of membrane phospholipids.