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蛋白酶的电化学研究

Electrochemical Study of Protein Enzymes
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摘要 电化学作为现代仪器分析重要的组成部分,在生命科学领域正扮演着越来越重要的角色.随着蛋白膜伏安法(protein film voltammetery,PFV)的不断完善,以及表面修饰技术、纳米技术、分子标记与分子识别技术等相关技术的不断发展,在蛋白酶研究领域,电化学正逐渐克服以往手段较为单一、研究目标不够多样、获取信息不够丰富等弊端.以本课题组在该领域所取得的一些研究成果为主,综述蛋白酶电化学研究的现状和发展趋势. Electrochemistry has become increasingly important in the study of life sciences. With the development of protein film voltammetry (PFV) and advances in surface modification, nanotechnology, molecular recognition and molecular marker, electrochemistry has overcome some drawbacks in protein enzyme studies. This article summarizes some typical work of the group, and reviews the progress in the electrochemical study of protein enzymes.
作者 李根喜
出处 《上海大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第4期567-572,共6页 Journal of Shanghai University:Natural Science Edition
基金 国家自然科学基金资助项目(90406005 20575028) 国家杰出青年科学基金资助项目(20925520)
关键词 蛋白酶 电化学 蛋白膜伏安法 protein enzyme electrochemistry protein film vohammetry (PFV)
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  • 1MACUS R A, SUTIN N. Electron transfer in chemistry and biology [J]. Biochim Biophys Acta, 1985, 811 ( 3 ) :265-322.
  • 2ARMSTRONG F A, HEERING H A, HIRST J. Reactions of complex metalloproteins studied by protein-film vohammetry [J]. Chem Soc Rev, 1997, 26(3):169- 179.
  • 3ARMSTRONG F A. Insights from protein film voltammetry into mechanisms of complex biological electron-transfer reactions [J]. J Chem Soc Dalton Trans, 2002, 5:661- 671.
  • 4CHEN K, HIRST J, CAMBA R, et al. Atomically defined mechanism for proton transfer to a buried redox centre in a protein [J]. Nature, 2000, 405(6788) :814-817.
  • 5ZHAO J, ZHU X, LI T, et al. Self-assembled muhilayer of gold nanoparticles for amplified electrochemical detection of cytochrome c [ J ]. Analyst, 2008, 133 (9) : 1242-1245.
  • 6ARMSTRONG F A. Protein film voltammetry: revealing the mechanisms of biological oxidation and reduction [ J]. Russ J Electrochem, 2002, 38 (1) :49-62.
  • 7FAN C, WANG H, SUN S, et al. Electron transfer reactivity and enzymatic activity of hemoglobin in a SP sephadex membrane [J]. Anal Chem, 2001, 73 ( 13): 2850-2854.
  • 8LIU X, ZHANG W, HUANG Y, et al. Enhanced electron-transfer reactivity of horseradish peroxidase in phosphatidylcholine films and its catalysis to nitric oxide [ J]. Journal of Biotechnology, 2004, 108(2) : 145-152.
  • 9LIU X, ZHENG X, XU Y, et al. Multi-step reduction of nitric oxide by cytochrome c entrapped in phosphatidylcholine films [ J ]. Journal of Molecular Catalysis B: Enzymatic, 2005, 33(1/2) :9-13.
  • 10LIU X, SHANG L, PANG J, et al. A reagentless nitric oxide biosensor based on hemoglobin/polyethyleneimine film [ J ]. Biotechnology and Applied Biochemistry, 2003, 38( 1 ) :119-122.

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