金黄色葡萄球菌诱导小肠上皮细胞炎性反应及凋亡作用的观察

Observation on Inflammatory Responses and Apoptosis of Caco-2 Cell Induced by Staphylococcus aureus

目的观察研究小肠上皮细胞Caco-2细胞对金黄色葡萄球菌感染的反应。方法采用细胞内菌落技术法推算Caco-2细胞内吞金黄色葡萄球菌的能力,采用Realtime-PCR法检测Caco-2细胞NOD2基因在金黄色葡萄球菌感染时的表达情况,采用ELISA、流式细胞术等方法观察Caco-2细胞在金黄色葡萄球菌感染时细胞因子分泌水平,核转录因子(nuclear factorκB,NF-κB)活化水平和细胞凋亡发生率。结果 Caco-2细胞能够内吞金黄色葡萄球菌,金黄色葡萄球菌感染可引起Caco-2细胞内NOD2表达增高,NF-κB活化水平增高,分泌细胞因子水平升高,Caco-2细胞发生凋亡,且凋亡率随感染后时间延长增高。结论金黄色葡萄球菌能够激活Caco-2细胞的免疫反应,细胞内模式识别受体NOD2在识别细胞内细菌、信号传导、核转录因子激活中发挥关键作用。

Objective To investigate the inflammatory responses of Caco-2 to Staphylococcus aureus.Methods By realtime-PCR to detect NOD2 gene expression of Caco-2 infected by S.aureus.Observe the cytokine secretion,activation of nuclear transcription factors,cell apoptosis of the Caco-2 cells infected by S.aureus using ELISA,flow cytometry etc.Results S.aureus can be internalized by Caco-2 cells.S.aureus infection of Caco-2 can cause increased expression of intracellular NOD2,the higher levels of cytokines secretion,stronger activation of NF-κB and Caco-2 apoptosis.Conclusion S.aureus can cause the inflammatory responses of Caco-2,NOD2 is a key component in intracellular pathogen recognition,signal transduction,activation of nuclear transcription factors in the process of internalization of S.aures by Caco-2.